破骨细胞相关因子在骨再建过程中表达的定量分析

Quantitative Study of Osteoclastic Related Factors in the Process of Bone Reconstruction

目的研究肿瘤坏死因子-α(TNF-α)、核因子-κB受体激动剂(RANKL)、抗酒石酸酸性磷酸酶(TRAP)在骨再建过程中的表达。方法用8周龄雄性C57BL／6J小鼠建立骨折模型,用RT-PCR法对骨形成过程中TNF-α、 RANKL和TRAP的表达进行时程分析。结果骨折后TNF-α、RANKL、TRAP的表达均上调,但峰值在不同的时间点出现。TNF-α在骨折后2 d达峰值,BANKL的峰值出现在骨折5 d后,而TRAP的表达在骨折后10 d达到峰值。结论骨的再建也有破骨细胞相关因子的参与,它有赖于成骨和破骨的精确平衡。

Objective To detect the expression of osteoclast related factors, tumor necrosis factor-α （TNF-α）, receptor activator of NF-kB ligand （RANKL） and tartrate-resistant acid phosphatase （TRAP）, in the process of bone remodeling. Methods 8-week-old male C57BL/6J mice were employed in this study to detect the expression of osteoclast related factors by real time PCR. Results TNF-α, RANKL and TRAP were up regulated in the process of bone remodeling, they reached the peak on day 2, 5 and 10 individually after injury. Conclusion Osteoclast related factors also participate in bone remodeling, which depends on the delicate balance between bone formation and bone resorption.