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PED/PEA-15对前列腺癌细胞凋亡的影响

The effect of PED/PEA-15 on prostate cancer cells apoptosis
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摘要 目的探讨PED/PEA-15在前列腺癌细胞(PC-3)中的表达及意义。方法用RT-PCR法检测PC-3中PED/PEA-15的表达,并扩增出PED/PEA-15全长基因,以pEGFP-N1为载体构建其真核表达质粒。以脂质体法转染PED/PEA-15真核表达载体至PC-3中,用流式细胞和MTT法检测PED/PEA-15对PC-3凋亡和生长的影响。结果PED/PEA-15在PC-3中表达。酶切和DNA测序证实PED/PEA-15的真核表达载体构建成功。转染PED/PEA-15真核载体的PC-3细胞凋亡下调,且对肿瘤杀伤因子KillerTRAIL的敏感性亦下降。结论抗凋亡因子PED/PEA-15可阻抑PC-3的凋亡,PED/PEA-15的表达对前列腺癌的发展有重要作用。 Objective： To study the effect of PED/PEA 15 on human prostate carcinoma cells （PC-3） apoptosis through detecting the expression of PED/PEA 15 and constructing its eukaryotic expressing vectors. Methods： The expression of PED/PEA 15 in PC-3 was detected by means of RT PCR technique. The gene of PED/PEA-15 was amplified by means of RT-PCR technique and was cloned into pEGFP-N1 vector. The construct of PED/PEA-15 was transiently transfected into PC-3 cells under induction of liposome. The apoptosis of cancer cells was deter mined by flow eytometry technique. Results：The expression of PED/PEA 15 was up-regulated in PC-3 cells. Enzyme digestion analysis and DNA sequencing confirmed that the PED/PEA-15 eukaryotic expression vector was constructed successfully. High expression of PED/PEA 15 can inhibit PC-3 cells apoptosis and degrade Kille- rTRAIL＇s proapoptosis effect. Conclusions： PED/PEA 15 can inhibit PC-3 cells＇ apoptosis, and the expression of PED/PEA-15 might be involved in prostate cancer＇s development.
作者 胡晓勇 陈晓春 余勇军 梁涛
机构地区 华中科技大学协和医院泌尿外科
出处 《临床泌尿外科杂志》 2006年第4期309-311,共3页 Journal of Clinical Urology
关键词 肿瘤标记物 前列腺肿瘤 细胞凋亡 PED/PEA-15 Prostate cancer Cell apoptosis
分类号 R737.25 [医药卫生—肿瘤]
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参考文献9

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临床泌尿外科杂志
2006年 第4期

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