圆盘状受体1在瘢痕疙瘩中的作用

Role of discoidin domain receptor 1 in keloid

目的:观察圆盘状受体1在瘢痕疙瘩不同部位的表达情况,进一步分析其在瘢痕疙瘩形成和发展中的作用。方法:选择2005-02/06在解放军第二军医大学附属长海医院门诊就诊的3例瘢痕疙瘩患者,均知情同意。瘢痕疙瘩事先均未进行任何治疗,将瘢痕疙瘩分为中央凹陷的老化部、隆起明显的增生部、表面潮红的浸润部及正常皮肤部。体外培养成纤维细胞,通过WESTBLOT实验检测瘢痕疙瘩不同部位圆盘状受体1、p53、基质金属蛋白酶表达情况,应用流式细胞仪检测细胞凋亡率。结果:①WESTBLOT实验检测结果:圆盘状受体1的表达以增生部、浸润部最明显,而老化部次之,正常皮肤部表达最弱。p53表达以老化部、增生部、浸润部为显著,正常皮肤部表达不显著。基质金属蛋白酶在老化部、增生部、浸润部表达强于正常皮肤部,而浸润部较老化部表达更强。②瘢痕疙瘩不同部位细胞凋亡率比较:正常皮肤部细胞凋亡率显著高于老化部及增生部[(10.1±3.5)%,(6.2±1.8)%,(5.1±3.7)%(P<0.05)],其中浸润部(4.6±3.4)%与正常皮肤部相比,差异具有极其显著性意义(P<0.01)。结论:在瘢痕疙瘩中表现出圆盘状受体1高表达及成纤维细胞低凋亡的特性,圆盘状受体1强表达区也有p53强表达,以上作用可保护细胞,防止细胞凋亡,而且有利于瘢痕疙瘩的浸润。

AIM： To observe the expressions of diseoidin domain receptor 1 （DDR1） in different parts of keloid and further explore its role in formation and progression of keloid. METHODS： Totally 3 keloid patients in Affiliated Changhai Hospital of Second Milirary Medical University of Chinese PLA from February to June 2006 were selected and all subjects knew and agreed with items. Untreated keloids were divided into several parts： ageing part with central foveas, proliferative part with obvious eminence, infiltrating part with flush surface and normal skin part. Samples were cultured in vitro into fibroblasts. Expressions of DDR1, p53 and matrix metalloproteinase （MMP） in different parts of keloid were detected with West blot method and apoptotic rate was determined by flow cytometry. RESULTS： ① Results of determination in West blot experiment： expressions of DDR1 were most significant in proliferative part and infiltrating part, then was the ageing part that in normal skin part was the weakest. Expressions of p53 were the most remarkable in ageing part, proliferative part and infiltrating part, while that in normal skin part was not marked. Expressions of MMP in ageing part, proliferative part and infiltrating part were stronger than that in normal skin part and that in infiltrating part was stronger than that in ageing part. ②Comparison of apoptotic rate in different parts of keloid： that in normal skin part was significantly higher than that in ageing part and proliferative part of keloid [（10.1±3.5）%,（6.2±1.8）%,（5.1±3.7）%, P〈 0.05]. There were remarkable significances in comparison between infiltrating part [（4.6±3.4）%] and normal skin part （P 〈 0.01）. CONCLUSION： There are expressions of DDR1 and low apoptotic characteristics of fibroblast in keloid and there are expression of p53 in strong expressive area of DDR1, which may protect fibroblast from apoptosis and is beneficial to infiltration of keloid.