摘要
目的:为了寻找慢性疲劳综合征的标志物,从分子水平上对慢性疲劳综合征进行诊断,运用运动实验的方法对慢性疲劳综合征患者外周血单核细胞基因表达进行观察。方法:实验于2005-07-10/08-10完成。①选择女性慢性疲劳综合征患者5例,作为试验组;健康女性5名,自愿参加本实验,作为对照组。两组在功率自行车上进行运动,强度控制在其最大负荷的70%左右。②在负荷前后抽取血液,分离单核细胞并提取总RNA,反转录成cDNA,进行信号标记后与带有3800个寡核苷酸片断的芯片进行杂交。③采用了分类对比法、分层聚类法、基因功能分类对比法来寻找基因表达差异及对功能归属进行了分析。结果:患者5例,健康者5名,均进入结果分析。①在负荷前后组内、组间的基因表达都有差异;实验组与对照组间的运动敏感基因表达的差异主要体现在与染色质合成、核小体、囊泡、细胞骨架以及膜蛋白受体功能有关的基因上。②与离子转运和离子通道活性相关基因表达方面,两组间的基准值在运动前就有明显差异,而在运动过后其差异变得更大。③在运动前后对对照组的运动应答基因进行了分析,结果发现有29条基因表达存在差异。结论:运动负荷与基因表达芯片相结合的方法在鉴定与慢性疲劳综合征相关基因的功能分类方面有着潜在的应用价值。
AIM: In order to find the signs of chronic fatigue syndrome and provide molecular diagnostic evidence for chronic fatigue syndrome, we observe the gene expression of monocytes in peripheral blood of women with chronic fatigue syndrome by using exercise method.
METHODS: The experiment was conducted from 10^th July to 10^th August 2005. ①Five female patients with chronic fatigue syndrome were chosen as experimental group; 5 female patients who were healthy were chosen as control group. They all had exercises on power bike, and the intensity was controlled at about 70% of the maximum load. ②We extracted their blood before and after their exercises, then isolated the monocytes and extracted total RNA, which were reversely transcribed into cDNAs, and were signed by Cy3 and Cy5 and hybridized with gene chip including 3800 oligonucleotide snippets. ③Methods of class comparing, hierarchical clustering and gene ontology comparison were used to analyze the difference of gene expression and gene function.
RESULTS: There were 5 patients and 5 healthy persons. All of them entered the stage of result analysis. ① Exercise-responsive genes differed between chronic fatigue syndrome group and controlled group. We judged differences in gene expression among and between two groups before and after exercises and evaluated differences in terms of gene ontology comparison. These. were genes classified in chromatin synthesis, nucleosome, cytoplasmic vesicles, membrane transport and G proteincoupled receptor. ②Differences in ion transport and ion channel activity were evident at baseline and were exaggerated after exercise. ③ We analyzed the exercise response gene before and after exercise in the control group and found that gene expression varied greatly in 29 pieces.
CONCLUSION: These results highlight the potential use of an exercises load combined with gene expression chip analysis in identifying gene ontologies associated with chronic fatigue syndrome.
出处
《中国临床康复》
CSCD
北大核心
2006年第16期107-109,共3页
Chinese Journal of Clinical Rehabilitation