新型人工心脏辅助装置材料组织炎症反应观察

Application of hard-segment modified PU in ventricule assisted device

目的观察用于制造人工心脏辅助装置的硬段相、软段相改性新型聚氨酯材料和未改性医用聚氨酯材料的组织炎症反应程度。方法选用材料硬度90A的硬段相改性医用聚氨酯(A组)、软段相改性医用聚氨酯(B组)和未改性医用聚氨酯材料(C组)组装成人工心脏辅助装置,建立犬心脏辅助转流模型。观察转流不同时间后心、肝、脾、肺、肾标本组织学变化,并测定不同转流时间后血液炎性因子(IL-6、IL-8、TNFα)含量。结果转流72 h后C组组织炎症充血、水肿,炎症反应明显强于A、B两组。48 h内体内IL-6、IL-8、TNFα炎性因子水平呈升高趋势,C组明显高于A、B组。48 h后各组炎性因子水平趋于稳定。结论硬段相改性医用聚氨酯材料比未改性的常见医用聚氨酯材料组织炎症反应轻微,具有良好的血液相容性,可作为人工心脏辅助装置的首选材料进一步推广应用。

AIM To observe the inflammatory reaction of 90-A consistency, soft-segment modified PU and pure PU, which were assembled into a ventricule assisted device （VAD）. METHODS The animal left ventricular experiment models were established with canines and VAD, which was made from hard-segment modified PU （group A）, soft -segment modified PU （group B） and pure PU （group C）. At different time points, histological changes were observed and inflammatory factors tites including IL-6, IL-8 and TNF-α were measured. RESULTS Group C had the worse inflammatory reaction compared with Group A and B. The levels of inflammatory factors of IL-6, IL-8 and TNF-α showed a light rising tendency within 48 hours and were higher in group C than those in group A and B （ P 〈 0.05 ）. The levels of these inflammatory factors stayed in a stable level after 48 hours of assisted circulation. CONCLUSION Hard-segment modified PU has only milder inflammatory reaction but better haemocompatibility than those of pure PU and can be the first choice of VAD material for wide application.