光动力联合5-FU治疗裸鼠直肠移植瘤的实验研究

Experimental study on transplanted rectal tumor of mice treated by photodynamic therapy combined with 5-FU

目的探讨增强光动力治疗(PDT)的有效途径。方法应用光动力和5-FU单独或联合对人直肠癌HR8348细胞株的裸鼠接种瘤进行治疗,观察移植瘤的生长情况及组织病理学改变,并测定肿瘤内脂质过氧化物丙二醛(MDA)的含量。结果光动力联合5-FU治疗对小鼠移植瘤的杀伤作用较单独应用光动力或单独应用5-FU的杀伤作用更明显(P<0.01),而且其MDA的含量明显高于其他组。结论PDT联合应用化学疗法可作为治疗肿瘤的一种方法。

Objective To explore the effectiveness of photodynamic therapy （PDT） combined with 5-FU on the treatment of transplanted rectal tumor in mice. Methods The nuke mice transplanted tumor of human rectal cancer HR8348 cells were treated with PDT alone, 5-Fu alone or PDT combined with 5-Fu respectively. The volume and weight of the tumors were measured and the histopathologic changes were studied. The level of malonaldehyde （MDA） in the tumors in different groups was analyzed. Results The lethal effect on transplanted rectal tumor was more obvious and the level of MDA was more higher in the PDT combined with 5-Fu group than those of other groups. Conclusion PDT combined with chemotherapy may serve as a method in treatment of tumor.