高血压大鼠肝阳上亢证的下丘脑双向凝胶电泳分析

Analysis of hypothalamuses in the spontaneous hypertension rats with hyperactivity of liver-yang using two-dimensional electrophoresis

目的通过双向凝胶电泳建立高血压肝阳上亢证大鼠与正常大鼠下丘脑分辨率高、重复性好的蛋白质表达图谱，并分析其差异蛋白质。方法用固相pH梯度双向凝胶电泳分离肝阳上亢证大鼠与正常大鼠下丘脑的总蛋白质，经考马斯亮兰染色，PDQuest 7．0软件分析。结果两种状态下的大鼠下丘脑图象平均匹配率达92％，且重复性较好，其蛋白质主要分布在等电点pI3-8，分子量Mr14．4～75kD范围。高血压肝阳上亢大鼠下丘脑约有777±27个蛋白质点，正常大鼠下丘脑约有780±31个蛋白质点。与正常大鼠比较肝阳上亢大鼠有22个点表达上升2倍及以上，有25个点表达下降2倍及以上。结论在肝阳上亢状态下有22个蛋白质表达明显增加，还有25个蛋白质表达明显减少。这些蛋白质的变化可能与高血压肝阳上亢的形成有关。

Objective To establish two-dimensional electrophoresis （2-DE） profiles with high resolution and reproducibility in analysis of hypothalamuses in the spontaneous hypertension rats （SHR） with hyperactivity of liver-yang to analyze the difference on the total proteins of hypothalamuses between the SHR and normal rats. Methods The total proteins of hypothalamuses in the rats were separated by immobilized pH gradient-based two-dimensional gel electrophoresis. With coomassie blue staining, the 2-DE image was analyzed by PDQuest 7.0 software. Results The average matching rate of the 2-DE images of hypothalamuses in the two kinds rats was 92% with good reproducibility. Proteins were mainly displayed at range of pI3 - 8, Mr （14.4～75） kD. The average spots in the SHR with hyperactivity of liver-yang and in the normal rats were 777±27 and 780±31 respectively. There were 22 spots of proteins up - regulated more than two times and 25 spots down - regulated more than two times in the SHR with hyperactivity of liver-yang than those of the normal rats. Conclusion There are 22 spots of proteins up - regulated and 25 spots of proteins down - regulated in the SHR with hyperactivity of liver-yang, which may be involved in the development of hyperactivity of liver-yang.