乙型肝炎病毒前C区/BCP区突变对血清HBV DNA含量的影响

Effects of HBV pre-core and basal core promoter mutations on serum HBV DNA level in chronic hepatitis B using Gene-chip technique

目的利用基因芯片技术检测乙型肝炎病毒(HBV)前C区/BCP区基因突变,探讨前C区/BCP区基因突变后血清HBV DNA水平的变化。方法应用基因芯片技术检测95例慢性乙肝的HBV前C区1896、1814及HBV C基因启动子(BCP)1762、1764四位点突变,同时用荧光定量PCR检测血清HBV DNA含量。结果95份血清标本中,有91份分别检测到了HBV前C区/BCP区基因突变,阳性率95.8%,其中G1896A突变33例(36.3%),A1762T+G1764A联合突变64例(70.3%),G1896A+A1762T+G1764A联合突变22例(24.2%),未检测到A1814C突变毒株。G1896A+A1762T+G1764A联合突变后,血清HBV DNA水平较未变异组降低(P<0.05),其它各组则无显著变化。结论应用基因芯片法可同时检测HBV多个突变位点。乙型肝炎病毒前C区/BCP区突变对血清HBV DNA含量有一定的影响。

Objective To investigate the clinical significance of HBV pre-core and basal core promoter mutations in chronic hepatitis B using Gene-chip technique. Methods Four spot mutations of 1896, 1814 dots in pre-core region and 1762, 1764 dots in basal core promoter region were detected in 95 patients with chronic hepatitis B using gene chips. Serum HBV DNA level was measured using fluorescent quantitation polymerase chain reaction （FQ-PCR）. Results In the 95 patients with chronic hepatitis B, HBV pre-core or basal core promoter mutations were detected in 91 patients, and the positive rate was 95.8%. The mutation rate of G1896A, A1762T associated with G1764A, and G1896A plus A1762T plus G1764A were 36.3%, 70.3% and 24.2% respectively. No A1814C mutant was detected in these patients. Compared with no mutation group, the serum HBV DNA quantity was lower in G1896A plus A1762T and G1764A mutation group, and no significant changes was found in other groups. Conclusion Several different variant sites of HBV can be detected at one time using gene chip technique. HBV pre-core and basal core promoter mutations have effect on the serum HBV DNA level in chronic hepatitis B patients to a certain extent.