电针对老年性痴呆大鼠海马神经元突触形态可塑性的影响研究

Effect of electroacupuncture therapy on morphological synaptic plasticity in hippocanipal neurons of rats with senile dementia

目的探讨电针治疗老年性痴呆的突触可塑性机制,为老年性痴呆的针灸作用机理研究提供新的思路。方法 SD大鼠随机分为对照组、模型组、假手术组和电针组;以穹隆-海马伞切断进行“老年性痴呆”造模,电针“百会”、“涌泉”、“太溪”、“血海”后,电镜观察海马CA3区突触形态可塑性指标突触数密度(Nv)、突触面密度(Sv)及平均面积(S)的变化。结果模型组大鼠的Nv(2．16± 0．17)、Sv(0．09±0．02)较对照组(6．16±0．96,0．27±0．05)明显减少(P<0．01);电针组大鼠的Nv(5．08±1．02)、Sv (0．23±0．04)较模型组明显增加(P<0．01)。模型组(0．20±0．03)大鼠S较对照组(0．04±0．01)明显增加fP<0．01); 电针组大鼠的S(0．06±0．01)较模型组明显减少(P<0．01)。结论电针“百会”、“涌泉”、“太溪”、“血海”具有一定程度的促进突触可塑性发挥的作用。

Objective To determine whether electroacupuncture （EA） therapy can enhance morphological synaptic plasticity in hippocampal neurons of rats with Alzheimer＇s disease （AD）. Methods Some aged male rats were randomly divided into control group, sham operation group, AD model group and EA group （n=6 per group）. AD models were built by cutting off the relation of fornix and hippocampal fimbria. EA therapy was conducted on such acupoints as ＂Baihui（DU20）＂, ＂Yongquan （KII）＂, ＂Taixi （KI3） ＂and ＂Xuehai （SP10）＂. The changes of synaptic plasticity in hippocampal CA3 region were observed by electron microscope. Results Compared with Nv and Sv in control group （6.16±0.96, 0.27±0.05）, the two parameters decreased in AD model group （2.16±0.17, 0.09±0.02, P〈0.01）, while increased in EA group（5.08±1.02,0.23±0.04, P〈0.01 ）. The S in EA group（0.06±0.01 ） decreased compared with AD group （0.20±0.03, P〈0.01 ）, which increased as compared with control group （0.04±0.01, P〈0.01）. Conclusion EA therapy on ＂Baihui（DU20）＂, ＂Yongquan （KI1）＂, ＂Taixi （KI3） ＂and ＂Xuehai （SP10）＂ can improve the morphological synaptic plasticity in AD rats.