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诱导免疫耐受治疗佐剂性关节炎的实验研究 被引量：4

The experimental research in the treatment of adjuvant arthritis with induced immunotolerance
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摘要 目的:探讨口服Ⅱ型胶原(CⅡ)对大鼠佐剂性关节炎(AA)的治疗作用,研究口服诱导免疫耐受过程中转化生长因子-β(TGF-β)表达的变化。方法:制备大鼠AA模型,观察口服CⅡ前后关节的肿胀度;采用ELISA法检测大鼠血清和关节浸出液中TGF-β的表达水平。结果:口服CⅡ可以明显减轻病变关节的炎症反应,降低发病率,推迟AA的发病并且使病程明显缩短。口服CⅡ诱导免疫耐受的AA大鼠血清和关节浸出液中TGF-β的表达明显高于未口服CⅡ的大鼠(P<0.05)。结论:口服CⅡ对大鼠佐剂性关节炎有明显的治疗效果;TGF-β是口服耐受治疗AA的重要细胞因子。 Objective：To investigate the therapeutic effect of oral administration of type Ⅱ collagen（C Ⅱ ） in the treatment of Wistar rats suffering from adjuvant arthritis（AA）,and to detect the expression of transforming growth factor-β（TGF-β） in the process of treating.Methods：The changes of articular swelling degree was observed in the AA rat models that had been given C Ⅱ by oral administration. Enzyme-linked immunoabsorbent assay（ELISA） was used to examine the expression of TGF-β in murine sera and articular leachates. Results： Oral administration of C Ⅱ could alleviated the inflammatory reaction of affected joints, reducded the incidence, delayed onset and obviously shorten course of the rats with AA. The expression of TGF-βwas remarkably increased in CⅡ orally tolerized rat models compared with control group. Conclusions： The therapeutic effect of oral administration of C Ⅱ on murine AA was obvious. TGF-β plays an important role in the treatment of AA rats by oral tolerance.
作者 陈柏松 徐玉东 马颜文 崔海山 钟淑琦 马月秋 赵太平
机构地区 哈尔滨医科大学解剖学教研室 牡丹江医学院解剖学教研室 广州医学院解剖学教研室
出处 《解剖与临床》 2006年第2期93-95,98,共4页 Anatomy and Clinics
基金 黑龙江省教育厅科技项目(No.10551146) 哈尔滨市留学回国人员基金(No.2003AFLXJ011)
关键词 口服耐受 佐剂性关节炎 Ⅱ型胶原 转化生长因子-Β Oral tolerance Adjuvant arthritis CⅡ TGF-β
分类号 R593.22 [医药卫生—内科学]
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参考文献16

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