强化训练对狗关节软骨的组织学影响

Effects of intensified training on histology in articular cartilage in dog

目的研究强化训练下狗膝关节软骨的组织学变化,以了解不同运动训练模式对膝关节软骨早期塑形改建的影响。方法20只实验用狗随机分为一般训练组(训练组,n=8)、强化训练组(强化组,n=8)和对照组(n=4),训练组与强化组分别以不同的训练方式进行训练。训练10周后所有实验动物均被施行安乐死,膝关节软骨行组织学检查。结果训练组和强化组训练10周时膝关节软骨有明显损伤及修复迹象,强化组关节软骨塑形改建情况优于训练组,但代表损伤程度的Mankin评分两组无明显差别。结论长时间大强度运动易产生软骨运动性损伤,修复过程也随之发生;强化训练较一般训练能更好地促进关节软骨的塑形改建。

Objective To study the adaptation process and extent of changes in articular cartilage in the canine knee joint to the intensifted training, in order to elucidate the influence of different movement training on remodeling and reconstruction of articular cartilag~ Metlmds Twenty adult dogs were randomly divided into three groups. Eight dogs were assigned to the short training group, and they were allowed to run on a slope of 10° with the speed of 200m/min. Eight dogs were assigned to short intensified training group, and they were allowed to run on a slope of 10° with a speed of 300m/min. Four dogs served as controls, and they were allowed to have unrestricted activity in the cage. Dogs in training group and intensified group were asked to, run for an hour both in the morning and in the aftemoon every day （tolally two hours per day）. The training lasted for ten weeks. All the dogs were euthanized after ten weeks, and all the knee joints were harvested and examined histologically. Results Histological examination after ten weeks of exereises demonstrated that the signs of cartilage damage and repair were obvious in canine knee joint in training group and intensified group. Remodeling process was bet- ter promoted in the intensified group compared with training group, but there was no significant difference in Mankin score between these two groups. Conclusion Movements for a long time with great intensity are prone to induce sport injury. It is followed by repair process. Intensified training promotes a better remodeling reconstruction of articular cartilage than ordinary training.