组织工程生物反应器及其仿生力学环境探讨

A Discussion on Tissue Engineering Bioreactor and Its Bionic Mechanal Environment.

组织工程生物反应器是在体外造就一个与体内相似的环境,加速培养细胞、组织乃至器官的系统,可广泛应用于如器官缺损患者替代、构建、保持或增强其组织功能,以及生物制药等领域。生物反应器作为组织工程中重要的功能型载体,已成为当前研究的热点。目前,生物反应器主要是从空间三维培养、生物力学、传质、培养环境(pH、PO2等)和物理因素(电场、磁场、超声)等方面开展研究。本文着重从力学环境的角度探讨了各种组织工程生物反应器,并在此基础上介绍了我们从仿生角度所分析、综合、设计的旋转壁式生物反应器,以及优化细胞生长力学环境的思路。

Bioreactor is a device which creates an environment in vitro similar to that in vivo, for culturing cells,tissues, even organs in tissue engineering, and can be applied to restore, maintain or enhance tissue function, as well as to the field of bio-pharmacy. Bioreactors have got attention as a very important carrier in tissue engineering. Currently, considering the knowledge of cell biology and engineering science, the research of bioreactor is mainly concentated in three-dimensional culture,biomechanics, mass transport, culture condition （ pH, PO2 etc） and physical factors （electric filed, magnetic field ,stress field） etc. In this paper,the mechanical environment of bioreactors for tissue engineering were discussed, and some new considerations based on new design of rotatingwall vessels bioreactor for optimizing the mechanical environment for cell growth were put forward.