子宫内膜与腹膜粘附过程中相互作用的研究

Adhesion of human endometrial fragments to peritoneum explants.

目的:通过建立人腹膜组织与子宫内膜组织共培养的离体实验模型,观察子宫内膜异位症早期粘附过程中相应的变化以探讨子宫内膜异位症的发生机理。方法:取前腹膜将其剪成5mm×5mm大,贴于预先包埋了鼠尾胶的24孔板中,取处理过的子宫内膜约15mg(湿重)滴在已经贴壁的腹膜组织上共培养。利用HE染色,免疫组化及扫描电镜进行观察,测定粘附情况。结果:(1)子宫内膜可以粘附在腹膜上,而且共培养1h就有粘附。增生期、分泌期的子宫内膜粘附率无差异;(2)粘附部位的子宫内膜组织中既有内膜腺上皮也有内膜间质细胞;(3)多数粘附部位没有完整的腹膜间皮细胞层。结论:子宫内膜腺上皮及间质细胞都参与了早期粘附过程,粘附率与子宫内膜的周期无关,在位内膜的特性可能与粘附率有关。

Objective：To evaluate the adhesion of human endometrial fragments to peritoneum explants in vitro. Methods： Explants of human peritoneum from the anterior abdominal wall were cultured with whole fragments of mechanically dispersed endometrium. Adhesion of endometrial fragments was studied by histologic examination and scaning electron microscopy. Results： （ 1 ） After incubation, both proliferative and secretory endometrial fragments were found attached to the peritoneum. There was no difference in the rates of attachment of the kinds of endometrium; （2） Hestain and immunohistochemical staining showed the attachment of both en- dometrial stroma and epithelial cell ; （3） Most of the implants did not have identifiable mesothelium beneath them. Conclusion：Endometrial epithelial and stroma cell can attach to the peritoneum. The characters of eutopic endometrium may be associated with endometriosis