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Human β-defensin2(hBD-2)在幽门螺杆菌相关性胃炎中的表达 被引量：2

Expression of human β-defensin 2(hBD-2) in Helicobacter pylori induced gastritis
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摘要 目的检测humanβdefensin2(hBD2)在幽门螺杆菌(Hp)相关性胃炎胃黏膜上皮中的表达,评价其在Hp感染的胃黏膜表面的抗菌作用。方法随机选择患者18例,取胃窦黏膜组织,以快速尿素酶及改良Giemsa染色两种方法判断Hp的存在与否。两者同时阳性诊断为Hp阳性,同时阴性为Hp阴性。18例患者中,Hp阳性10例,阴性8例。另以RTPCR法检测标本中hBD2的表达。结果10例Hp阳性标本中8例呈现hBD2mRNA的高表达,1例呈现低表达,总表达率90%;8例Hp阴性标本中6例未检测出hBD2mRNA,2例呈现hBD2mRNA的表达,表达率25%。结论Hp感染可诱导hBD2在胃黏膜上皮中的表达。体外试验表明hBD2抑制Hp的生长,推测hBD2在Hp相关性胃炎中起到一定的抗菌作用。 Objective To detect the expression of human β-defensin 2（hBD-2） in Helicobacter pylori induced gastritis and to evaluate the antibacterial role of hBD-2 against H. pylori infection in gastric mucosa. Methods Biopsied gastric mucosa specimens from H. pylori positive（ n=10） and H. pylori negative（ n=8） individuals were used. H. pylori was detected by urease test and histological examination. Expression of hBD-2 were examined by reverse transcripfion-polymerase chain reaction （RT-PCR）. Results Eighty percent of H. pyloritive specimens expressed a high level of hBD-2 mRNA while 25% H. pylori negative specimens expressed hBD-2 mRNA. Conclusion H. pylori infection induced the expression of hBD-2 in gastric mucosa. It is suggested that hBD-2 plays an antibacterial role in H.pylori induced gastritis.
作者 姜葵 王邦茂 刘心娟 阎雪燕 马晴 张洁
机构地区 天津医科大学总医院消化科
出处 《中华微生物学和免疫学杂志》 CAS CSCD 北大核心 2006年第3期255-257,共3页 Chinese Journal of Microbiology and Immunology
关键词 HUMAN β-defemin 2 幽门螺杆菌 胃炎 Human β-defensin 2 Helicobacter pylori Gastritis
分类号 R573.3 [医药卫生—消化系统]
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参考文献10

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共引文献2

同被引文献20

  • 1段德鉴,蒋献,冉玉平,李福民,吴琦,周光平.白念珠菌对角质形成细胞β防御素-2 mRNA表达的影响[J].中华皮肤科杂志,2005,38(4):202-204. 被引量：10
  • 2Karlheinz Kiehne,Gabriele Brunke,Franziska Wegner,Tomas Banasiewicz,Ulrich R F lsch,Karl-Heinz Herzig.Defensin expression in chronic pouchitis in patients with ulcerative colitis or familial adenomatous polyposis coli[J].World Journal of Gastroenterology,2006,12(7):1056-1062. 被引量：8
  • 3李绍强,周奇,梁力建,彭宝岗,华赟鹏,吴宝安.肝细胞癌患者树突状细胞免疫功能的研究[J].中国病理生理杂志,2007,23(1):124-126. 被引量：2
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中华微生物学和免疫学杂志
2006年 第3期

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