N-乙酰胺基乙酸甘草次酸酯的合成及HPLC检测

Studies on Synthetic Methods of N-Acetyl Glycine-glycyrrhetinic Acid Ester by HPLC

甘草次酸（glycyrrhetinic acid，2）是从豆科植物甘草的根茎中提取得到的五环三萜类化合物。药理活性研究表明：2的3-羟基被取代后，其抗炎活性、抗溃疡活性较为显著，且3、30-位分别成酯后具有较高的抗炎活性。

N-Acetyl glycine-glycyrrhetinic acid ester was synthesized by N-acetyl glycine and glycyrrhetinic acid in CH2Cl2 in the presence of DCC/DMAP under three different conditions respectively： stirring at 18℃, refluxing at 40℃, refluxing at 40℃ under ultrasonic radiation. The reaction speed of esterification was detected by HPLC. The results showed that the yield of ester was higher under the last condition in shorter reaction time.