高效液相色谱法测定复脉定颗粒中阿魏酸含量

Determination of ferulic acid in Fumaiding grannule with HPLC

目的:应用高效液相色谱法测定复脉定颗粒中阿魏酸的含量。方法:采用DiamonsilTMC18色谱柱(5μm,250mm×4.6mm),流动相为乙腈-0.5%冰醋酸水溶液(20∶80),流速1.0mL/min,检测波长313nm。结果:阿魏酸在进样量0.232￣1.392μg范围内线性关系良好(r=0.9999);平均加样回收率为98.54%,RSD=0.58%(n=6)。结论:本法操作简便、准确,灵敏度高,重现性好,可用于复脉定颗粒中阿魏酸的含量测定。

Objective： To establish an HPLC method for determination of ferulic acid in Fumaiding granule. Methods： Stationary phase was Diamonsil^TM C18 column （5 μm, 250 mm×4.6 mm）; mobile phase was acetonitrile-0.5% acetic acid glacial （20：80）; flow rate at 1.0 mL/min; UV detection wavelength was 313 nm. Results： The ferulic acid was linear within the range Of 0.232 ^-1.392μg （r = 0.9999）, and the average recovery rate was 98.54%, with the RSD of 0.58%（n = 6）. Conclusions： The method is simple, accurate, highly sensitive and reproducible. It may be used for the quantitative determination of ferulic acid in Fumaiding grannule.