高效复合菌在木薯酒精废液处理中的应用研究

Research and application of dominant bacteria in treatment of alcohol wastewater

对酒精废液生物处理反应器内颗粒污泥中的微生物进行分离纯化。分离出的8株高效优势菌,初步鉴定结果:1号为皮杆菌属;2号为棒杆菌属;3号、4号为微小杆菌属;5号为乳杆菌属;6号为纤维单胞菌属;7号为丙酸杆菌属;8号为红长命菌属。对8种纯化后的菌株进行混合培养,确定了复合菌群生长最佳培养温度为37℃,最适pH值为6.8。在处理过程中投加适量葡萄糖、硝酸铵和微量元素能够提高复合菌群的处理效果。最佳添加量为:葡萄糖0.5g/L,硝酸铵1g/L,Fe2+5mg/L和Ca2+25mg/L。在日处理量100m3的UASB生物反应器中投加该复合菌群后,木薯酒精废液的COD去除率明显提高,达到90%以上。处理系统运行稳定。

The present paper aims to introduce the authors＇ research on the dominant bacteria in the treatment of alcohol wastewater. We have found that there are 8 strains of bacteria which can be separated from the alcohol wastewater bioreactor sludge and have proper adaptability and high ability of degradation. Such bacteria that we have preliminary identified are： dermabacter （No. 1）, corynebacterium （No. 2）, exiguobacter（No. 3 and No. 4）, lactobacillus（No. 5）, cellulomonas （No. 6）, propionibacterium （No. 7） and rubrivivax （No. 8）. Cultivating the above said 8 bacteria mixed and then confirming the optimal cultivating conditions are PH6.8 and temperature 37 ℃. Having analyzed the influence of C, N resources and microelements on the degradation capability of the compound bacteria, the results show that adding suitable glucose and microelement can obviously improve the degradation ability of the compound bacteria. The optimum added quantity is glucose 0.5 g/L, NH4NO3 1 g/L, Fe^2＋ 5 mg/L, Ca^2＋ 25 mg/L. Applying the compound bacteria to the UASB bioreactor which has 100 m^3 disposal ability per day, the system will be able to operate very stably and the COD removing rate will be able to get enhanced and reach 90% .