食管癌患者CD_(44)V_5和Nm23-H_1的基因表达和病理学特征及其与术后生存的关系

The prognostic value of histopathological features and expression of CD_(44)V_5 and Nm23-H_1 gene products in esophageal cancer

目的探讨CD44V5和Nm23-H1基因表达及病理学特征与食管癌患者术后生存的关系。方法应用免疫组织化学S-P法对50例随访资料完整的食管癌标本进行CD44V5和Nm23-H1的基因产物测定,结合病理学特征与患者生存期进行Kap lan-M e ier单因素和Cox回归多因素模型分析。结果Kap lan-M e ier分析显示,食管癌患者的年龄、病理分级、临床TNM分期、CD44V5表达、Nm23-H1表达、侵袭深度及淋巴结转移是影响患者预后的重要因素(P<0.05);Cox回归多因素模型分析显示,肿瘤的病理分级[Exp(B)=3.8588]、TNM分期[Exp(B)=1.5927]、CD44V5[Exp(B)=6.1325]、Nm23-H1[Exp(B)=0.2766]表达是独立的预后因素,其中以病理分级、CD44V5和Nm23-H1表达最为重要。结论食管癌的病理分级、CD44V5、Nm23-H1表达和TNM分期均为独立的预后因素,其中CD44V5是危险因素,而Nm23-H1是保护因素。

Objective To investigate the prognostic value of various hiatopathological features and expression of CD44V5 and Nm23-H1 gene products in esophageal cancer （EC）. Methods The tumor samples from 50 primary EC patients were evaluated for following parameters ： the age, sex, tumor size, depth of invasion, pathological type, histopathological classification, TNM stage, lymph node metastasis, CD44V5 and Nm23-H1 expression. The prognostic impacts of these parameters were analyzed by univariate and multivariate survival analyses. Results The univariate Kaplan-Meier analysis showed that the age, histopathological classification, TNM stage, depth of invasion, lymph node metastasis, CD44V5, Nm23-H1 expression （P 〈 0.05, respectively） were very important for prognosis of EC patients. The multivariate Cox analysis demonstrated that only histopathological classification, TNM stage, CD44V5 and Nm23-H1 expression were significant prognostic factors （ P 〈 0.05, respectively）, especially histopathological classification, CD44V5 and Nm23-H1 expression. Conclusion For EC patients, histopathological classification, TNM stage, CD44V5 and Nm23-H1 expression are independent prognostic factors as well as the TNM stage, espe- cially CD44V5 expression was risk factor, and that Nm23-H1 expression was protective factor.