PI3K/AKT抑制剂对人肺癌细胞株生长的影响

The Effect of the Phosphatidylinositol 3'-Kinase-AKT Inhibitor on Proliferation of Lung Carcinoma Cell Lines

目的研究PI3K/Akt特异性抑制剂LY294002对人肺腺癌细胞株(A549)增殖的影响。方法用免疫细胞化学S-P法检测对照组和LY294002干预组A549细胞P-Akt蛋白的表达,MTT法检测对照组和LY294002干预组(LY294002 5μmol/L,10μmol/L,20μmol/L,40μmol/L)干预24h,48h7、2h后A549细胞增殖的抑制率。结果LY294002以剂量、时间依赖性方式抑制A549细胞的生长,各个LY294002干预组与对照组相比P-Akt蛋白的表达降低,有统计学意义(P<0.01)。结论LY294002对人肺腺癌A549细胞有抑制生长的作用。LY294002对P-Akt表达的抑制可能是细胞增殖受抑的重要原因。

Objective To study the effect of LY294002 on inhibiting the proliferation of human lung adenocarcinoma cell lines A549. Methods The A549 cells were treated by LY294002 20 μmol/L for 24 h, lmmunohistochemistry was used to detect the expression of P-Akt in A549 cell lines, MTT reduction assay was used to evaluate the inhibitory rate of A549 cells treated by LY294002 （LY294002 5 μmol/L, 10 μmol/L,20 μmol/L,40 μmol/L） for 24 h,48 h and 72 h.Results The proliferation of A549 cells was inhibited in some time and dose dependent patterns by LY294002,P-Akt protein expression of A549 cells was inhibited by LY294002 （ P 〈 0.01 ） .Conclusions LY294002 can inhibit the proliferation of human lung adenocarcinoma cell lines A549, the inhibitive effect of LY294002 on P-Akt expression may be an important cause of cells proliferation inhibition in human lung adenocarcinoma cell lines A549