恶性淋巴瘤患者的染色体畸变

Chromosome Abnormalities of Malignant Lymphoma

目的为了探讨恶性淋巴瘤(ML)染色体改变的规律及其与组织学分型的相关性。方法采用改良短期培养法制备24例ML患者的外周血染色体标本。结果24例ML进行了细胞遗传学研究,23例获得满意分裂相。所获得的ML患者的外周血染色体均显示不同程度的数目和(或)结构克隆异常,多涉及1,2,3,5,6,8,13,14,15,17,18,21号及X等染色体改变。不同的染色体畸变与ML的组织学分型有关。NHL的染色体众数以超二倍体、亚二倍体居多,HD以高异倍体、超二倍体、假二倍体居多。多种染色体结构改变涉及17p、1p、1q、2p、2q,提示这些部位的丢失或断裂重排与ML的发生有关。结论细胞遗传学与组织病理学结合起来,有助于ML的分类,并对探讨ML的发生机制、临床诊治和预后判断具有一定的意义。

Objective To explore the relativity of chromosome abnormalities in malignant lymphoma （ML） and its relevance to histopathology. Methods The cytogenetic study on 24 patients with ML by the methods of short term culture to prepare the chromosome from peripheral blood. Results Mitotic cells that could be used for analysis were found in 23 cases, h showed that the numerical and/or structural abnormalities of clone chromosome were found in all patients with ML and were often involved in chromosomes 1,2,3,5,6, 8,13,14,15,17,18,21 and X. The chromosome aberrations were associated with the histopathological type of ML in some extent. The hyperdiploid and hypodiploid of chromosome were mostly seen in patients with Non-Hodgin＇s lymphoma（NHL）, whereas the polyploid and hyperdiploid and pseudodiploid were mostly seen in Hodgkin＇s disease（HD）. Many structure aberrations of chromosome involved in 17p, 1 p, 1 q,2p ,2q, which suggested that the structure deletion and breakpoint region should be associated with ML. Conclusions The combination of the cytogenetic and pathologic study could be benefit to classification, pathogenesis, diagnosis, treatment and prognosis of ML.