摘要
目的探讨肝病患者血清中是否出现自身抗体及该抗体与疾病的关系.方法收集肝病患者血清,以HEP-2细胞、鼠肝和猴肝组织切片和可溶性肝抗原为抗原基质,用间接免疫荧光法、蛋白质印迹法检测肝病患者血清中的自身抗体.结果①间接免疫荧光法检测自身抗体显示:慢性肝炎、肝硬化、肝癌三组阳性率分别为22.3%(29/130),30.9%(30/97),34.1%(14/41),它们之间无显著性差异(P>0.05),但三组阳性率与急性肝炎(7.0%)、正常对照组(1.5%)比较有显著性差异(P<0.01).②蛋白质印迹法检测肝特异性自身抗体靶抗原时发现慢性肝炎、肝硬化患者约66.0%,的抗原相对分子质量多集中在47kD及52kD上,两组抗原条带分布相似,有30.0%的肝硬化、肝癌患者在20kD及32kD分子质量之间的阳性条带上、下处多出现1~2条无对应条带.结论慢性肝炎、肝硬化、肝癌患者血清中自身抗体的阳性率明显高于急性肝炎和正常对照组.患者血清中存在抗肝组织自身抗体,其相对应的靶抗原有多种,如能进一步鉴定靶抗原有望成为诊断肝脏疾病的免疫学标志物.
ObJeetive To study the change of auto-antibody and the relationship between the change and the course of liver disease. Methods Auto-antibodies in serum of patients with liver disease were identified by indirect immune fluorescence (IIF) and western blot (WB) ,for which the applied antigens were HEP-2 cells, rat liver tissue and monkey liver tissue. Results No significant difference was noted for ANA in three types of diseases ,chronic hepatitis 22.3 % (29/130) ,hepatoeirrhosis 30. 9% (30/97) and liver cancer 34.10% (14/41),but there were notable differences between them and acute hepatitis (7.0%) and normal people (1.5%) respectively. Bands were similar in chronic hepatitis and hrpatocirrhosis when extracted nuclear antigens were tested by W'B. the main molecular bands at 47 kD and 52 kD. There were 1 - 2 different bands at 20 -32 kD between hepatoeirrhosis and liver cancer in 30. 0% patients. Conclusion Auto-antlbody in patients with chronic hepatitis ,hepatocirrhosis and liver cancer was significantly higher than those in patients with chronic hepatitis and in normal persons. Auto-antibody is related to the progress of liver diseases and may be immunological marker for the diagnosis of them.
出处
《现代检验医学杂志》
CAS
2006年第2期70-72,共3页
Journal of Modern Laboratory Medicine
关键词
自身抗体
慢性肝炎
肝硬化
肝癌
auto-antibody
chronic hepatitis
bepatocirrhosis
hepato carcinoma