钝齿棒杆菌精氨酸生物合成相关基因簇argCJBDFR的扩增及序列分析

Cloning and sequencing of the tandem arginine biosynthetic genes argCJBDFR from Corynebacterium crenatum A.S1.542.

采用PCR方法扩增钝齿棒杆菌精氨酸生物合成基因簇argCJBDFR,其序列长为6080bp,其中含有argJ、argB、argD、argF和argR5个完整的开放阅读框。序列和结构分析表明这5个结构基因编码的氨基酸序列与谷氨酸棒杆菌的相应基因编码的氨基酸高度同源,同源性分别为92%、95%、96%、97.5%和100%。

The tandem arginine biosynthetic genes argCJBDFR from Corynebacterium crenatum were amplified by PCR. Sequence analysis indicates that there are five open reading frames, including argJ, argB, argD, argF and argR in the 6080bp stretch. The amino acid sequences coded by argjBDFR genes show 92%, 95%, 96% , 97. 5% and 100% identity to those of Corynebacterium glutacimum, respectively.