利用植物体细胞无性系变异技术选育高粱恢复系“011”的研究

A restorer line '011'for hybrid sorghum production developed by using somaclonal variation technique.

试验研究以高粱恢复系“0-30”幼穗作外植体,用10Gy60Co辐照处理,离体培养得到89株再生苗(R0)。R1代株系27.9%出现7种可见性状的变异,从第2代(R1)中筛选出稳定或分离的优良变异株,经多代选择最终选育出高粱恢复系“011”,用其配制的杂交种“7050A/011”产量高且抗病性强。

The young infloresenees collected from ‘0-30＇ , the male parent of ‘Shenza 5＇ ,were exposed to ^60Co-γ rays at a dose of 10Gy, then in vitro cultured on MS media. A total of 89 R0 regenerants were obtained. Solid and segregating somaelonal variations in seven kinds of visual traits were seen in RI generation with a variation frequency of 27.9%. Elite variants were isolated from the R1 solid and segregating population and crossed to sorghum male sterile lines. A somaclonal restorer called ‘011＇ were selected out. The hybrid combination ‘ 7050A/011＇ was featured by its high production and high antifungal.