亚砷酸钠对人淋巴细胞增殖抑制机制的实验研究

Experimental studies on mechanism of the proliferation inhibition in human lymphocytes induced by sodium arsenite

目的探讨亚砷酸钠(NaAsO2)对人淋巴细胞体外增殖抑制的影响。方法用不同浓度的NaAsO2处理细胞后,通过噻唑蓝还原法,吖啶橙荧光染色法和流式细胞术观察NaAsO2对淋巴细胞增殖的影响。结果NaAsO2低、中、高剂量对淋巴细胞增殖有抑制作用,荧光染色法测得凋亡率分别为10.07%、18.79%、28.35%。结论NaAsO2抑制淋巴细胞增殖,可能与其诱导凋亡有关。

Objective To explore the effect of sodium arsenite （ NaAsO2 ） on the proliferation of human lymphocytes in vitro. Methods After the lymphocytes were treated with differem concentrations of NaAsO2 in vitro, changes of cell proliferation were observed with MTT method, acridine orange（AO） of fluorescent light staining and flow cytometry. Results The proliferation function of lymphocytes was inhibited at the doses of 0. 1 - 5. 0 μmol/L NaAsO2, and fluorescent light staining showed that the apoptosis rates of NaAsO2-treated lymphocytes were 10. 07%, 18.79% and 28.35% respectively. Conclusion It was deduced that the proliferation inhibition of lymphocytes induced by NaAsO2 could be associated with apoptosis in these cells.