正己烷对制鞋业工人白细胞趋化性影响的研究

Study on the effects of polymorphonuclear leukocyte chemotaxis in shoe workers exposed to n-hexane

目的研究正己烷对人类白细胞趋化性的影响,探讨白细胞趋化性作为正己烷效应性生物标志物的可行性。方法用活性炭吸附采集法采集工作场所正己烷,并用热解析-气相色谱法检测工作场所空气中正己烷的浓度;班后采集工人静脉血并用肝素抗凝,用琼脂糖板扩散法测定各岗位工人的白细胞趋化性。结果正己烷对人白细胞趋化性有明显的影响,随工人接触正己烷浓度升高,白细胞抑制率呈下降趋势,当接触正己烷空气浓度为92.5mg/m3时,白细胞趋化距离为(1.08±0.22)mm,趋化抑制率为28.1%,趋化性明显受到抑制(P<0.05)。结论白细胞趋化性可尝试作为正己烷效应性生物标志物,但仍需进一步深入研究。

Objective To study the effects of polymorphonuelear leukocyte ehemotaxis in human exposed to n-hexane. The feasibility of using polymorphonuclear leukocyte chemotaxis as the effect biomarker of n-hexane was also discussed. Methods n-Hexane was collected by activated charcoal in the workplaces and detected by pyrolysis-gas chromatography. Polymorphonuclear leukocyte was extracted by venous blood in the off-duty workers. Polymorphonuclear leukocyte chemotaxis was analyzed by mea suring diffusivity on gelose plates. Results Polymorphonuclear leukocyte chemotaxis was restrained in workers exposed to nhexane. With the concentration of n-hexane increasing, leukocyte chemotaxis reduced accordingly. When the concentration of nhexane was 92. 5 mg/m^3 , the distance of polymorphonuclear leukocyte chemotaxis was （ 1.08 ± 0. 22） mm, the rate of polymorphonuelear leukocyte ehemotaxis was 28. 1% and polymorphonuelear leukocyte chemotaxis was restrained sigmifieantly （P 〈 0. 05 ）. Conclusion Polymorphonuelear leukocyte ehemotaxis might be the effect biomarker of n-hexane but needs to be explored father.