邓恩桉叶挥发性成分的提取及分析

Extraction and Determination of Volatile Constituents of Leaves from Eucalyptus dunnii

采用水蒸气蒸馏法从邓恩桉叶中提取挥发油中的油相成分,用乙醚作为溶剂从蒸馏残液中萃取挥发油的水溶性物质,并利用气相色谱-质谱(GC-MS)联用分析比较二者的化学成分。邓恩桉叶挥发油油相部分的得油率为3.67%(以干重计),其中确认了52个组分,占油相部分总量的97.04%,油相部分主要成分为1,8-桉叶油素(24.71%),其次为香树烯(9.10%)、γ-松油烯(8.87%)、α-蒎烯(7.67%)等;水溶性成分的得油率为0.85%(以干重计),其中确认了36个组分,占水溶性部分总量的83.52%,香树烯(19.40%)和蓝桉醇(15.67%)是水溶部分的主要成分。

The volatile constituents of leaves from Eucalyptus dunnii, including oil fraction and water soluble fraction, were extracted and determined. Oil fraction of volatile components was obtained through steam distillation. Ether was used as a solvent to extract the water-soluble fraction of the volatile compounds from the left liquid after steam distillation in order to know the quantity and constituents of volatile compounds dissolved in the water phase. To the oil fraction,yield of oil was 3.67% （dry weight basis） ,and to the water-soluble fraction,it was 0.85 % （dry weight basis）. Both oil fraction and water-soluble fraction were analyzed by gas chromatography-mass spectrometry method. The results showed that 52 compounds that were 97.04% of the oil fraction were identified and 36 compounds that were 83.52% of the water-soluble fraction were identified. The major constituents in oil fraction were 1,8-cineole （24. 71 % ）, followed by aromadendrene （9. 10 % ）, γ-terpinene （8.87 % ）, α- pinene （7.67%）. Aromadendrene （19.40%） and globulol （15.67%） were found to be the major compounds in the water-soluble fraction.