青钱柳种子次生休眠的发生及贮藏物质的变化

Induction of the Secondary Dormancy and Metabolism Changes of Storage Substances in Cyclocarya paliurus

以低温吸胀的青钱柳种子为材料,采用酸蚀、GA3浸种或GA3拌沙层积等措施进行处理后,对种子的萌发情况和层积过程中贮藏物质的变化进行研究。结果表明:种子成熟脱落后经历的冬季雨雪作用已部分或完全解除了它的初生休眠,人为地中断冷层积及其他因素综合作用可能导致种子进入次生休眠。酸蚀后在(23±2)℃条件下层积的种子发芽率仅为10.3%,而酸蚀后又经GA3浸种和GA3拌沙低温层积的发芽率可提高到44.7%。结果还显示,层积过程中种子三大贮藏物质呈缓慢下降趋势,但各自的分解酶活性并不完全随着贮藏物质含量的降低而增强,尤其是脂肪酶呈现不断下降的趋势。

The study was to investigate the differential responses of germination and the changes of the storage substances during the stratification in Cyclocarya paliurus seeds imbibed in cold woodland. In the present study, the seeds were treated by the acid scarification, GA3 soaking or cold stratification. It was found that the primary dormancy of the seeds was relieved partially or entirely because they were under the natural environments such as rain, snow and so on. These seeds were suspended from the cold stratification, thus the primary dormancy became greater and even they entered the secondary dormancy owing to the integrated effects＇ of all treatments. The induced rate of the secondary dormancy differed from the treatments and the incubation conditions. The germination percentage of seeds stratified in （23-±2）℃ was 10.3%, while it was up to 44.7% after the acid scarification, GA3 soaking and cold stratification. The content of storage substances decreased slowly. However, the catabolism of storage substances was not very consistent with the changes of their enzymes, particularly in the lipase. It might be relative to the secondary dormancy.