大网膜脂质血管生长因子提取及功能的初步研究

Preliminary Studies on Analysis and Function of Omentum Lipid Angiogenic Factor

应用Goldsmith报道的方法稍加改进,建立了从人及兔大网膜中抽提脂质血管生长因子（LAF）的流程,发现不管是人或兔的LAF均可使兔角膜形成新生毛细血管网,而皮下脂肪抽提物及PBS均无此作用。将大网膜和皮下脂肪抽提物同时作高效薄层层析（HPTLC）,发现大网膜抽提物中有一特异条带,不同于皮下脂肪抽提物,紫外光谱分析表明其吸收峰在250 nm。还研究了LAF对小鼠胸腺细胞增殖及对PHA诱导IL-2产生的影响,结果表明具有抑制作用且呈剂量依赖关系。

In this paper, we established the method for extracting lipid angiogenic factor from human and rabbit omentum. The angiogenesis created by this material was observed in the rabbit cornea butnot by subcutaneous fat extract and PBS as control. HPTLC results showed that omen turn extraction has a specific band which is different from subcutaneous fat extraction. Analysis of UV spectrum demonstrated that the absorbance is about 250 nm. We also found that LAP can significantly suppress the mice thymocyte proliferation and ConA-induced IL-2 production