胃癌细胞凋亡相关基因、PCNA与细胞凋亡的关系

目的探讨细胞凋亡相关基因、增殖细胞核蛋白(PCNA)与胃癌细胞凋亡的相关性。方法采用原位末端标记法及免疫组化技术,检测115例胃癌及癌前病变患者和正常人胃黏膜上皮细胞凋亡指数bcl-2、bax、PCNA的表达。结果正常人、浅表性胃炎、萎缩性胃炎、不典型增生、胃癌的细胞凋亡指数分别为(4.58±1.92)%、(10.14±2.56)%、(24.6±35.19)%、(19.35±4.04)%、(19.81±4.66)%;bcl-2蛋白表达分别为0、18.5%、20.3%、44.6%、58.5%;bax蛋白表达分别为20.4%、51.5%、55.2%、27.6%、16.8%;PCNA分别为(3.93±1.02)%、(13.58±4.65)%、(20.16±4.89)%、(24.30±4.44)%、(39.79±6.37)%、(54.79±10.6)%。结论胃黏膜癌变过程中,起初三个阶段细胞凋亡指数、bcl-2、bax和PCNA同时增加,而至萎缩性胃炎后,细胞凋亡指数、bax表达显著减少,bcl-2、PCNA表达显著增加,说明以上因素在胃癌演变过程中可能起重要作用。

Objective： To investigate the correlation between cell apoptosis related genes, PCNA and gastric cancer carcinogenesis and progression. Methods： By using tunel technique and immunohistochemistry methods, the cells apoptosis, bcl-2, bax and PCNA indexes were examined in 115 cases of gastric cancer, precancerous lesions and the normal controls. Results： In the normal group, chronic superficial gastritis group, atrophic gastritis group, atypical hyperplasia group and gastric carcinomas group, the apoptosis indexes were （4.58±1. 92）%, （10.14＋2.56）%, （24.6±35.19）%, （19.35±4.04）% and （19. 81±4. 66）% respectively; bcl-2 were 0, 18.5%, 20. 3%, 44.6% and 58.5% respectively; bax were 20.4%, 51.5%, 55.2%, 27.6% and 16.8% respectively; PCNAindexes were （3.93±1.02）%, （13.58±4.65）%, （20. 16±4.89）%, （24. 30±4. 44）%, （39.79±6.37） % and （54.79±10.6）% respectively. Conelusion：The results indicate that the apoptosis, bal-2, bax and proliferating indexes increase simultaneously in normal, chronic superficial gastritis and atrophic gastritis; However, apoptosis indexes and bax decrease significantly, bcl-2 and PCNA indexes increase continuously in atypical hyperplasia and carcinoma. These results further suggest that they possibly play an important role in the pathogenesis of gastric carcinoma.