化疗药物对胆管癌细胞的敏感性及PTEN蛋白表达的影响

目的探讨化疗药物体外对胆管癌细胞系QBC 939作用的敏感性及对PTEN蛋白表达的影响。方法采用M TT法和流式细胞术检测常用的六种化疗药物体外对胆管癌细胞株QBC 939的敏感性,并运用W est-ern B lot法检测药物体外作用于胆管癌细胞系QBC 939后PTEN蛋白的表达。结果六种化疗药物对于胆管癌细胞的敏感性依次为5-氟脲嘧啶、丝裂霉素、长春新碱、阿霉素、顺铂、氨甲蝶呤。与对照组比较,六种化疗药物体外作用于胆管癌细胞系QBC 939 48h后,PTEN的表达量有不同程度的增高。结论化疗药物体外作用于QBC 939细胞系,可通过上调PTEN的表达最终达到抗癌的作用。

Objective： To investigate the effect of chemotherapeutic drugs on the expression of cholangiocarcinoma QBC939 in vivo and the expression of the tumor suppressor gene PTEN. Methods： By using MTT method, cytofluorometry and Western Blotting assay, the sensititvity of 6 kinds of chemotherapeutic drugs were detected, and PTEN protein expression was examed in cholangiocarcinoma cell line QBC939 after the administration of 5-FU, MMC, DDP, VCR, ADM, MTX. Results： Six kinds of chemotherapeutic drugs＇ sensitivity was arranged from high to low as following： 5-FU, MMC, VCR, ADM, DDP, MTX. Conclusion： Chemotherapeutic drugs play anti-cancer roles in cholangiocarcinoma cell line QBC939 by significantly upregulating PTEN protein.