bcl-2反义寡核苷酸与VP16协同抑制小细胞肺癌细胞增殖的研究

目的研究bcl-2反义寡核苷酸与足叶乙甙(VP 16)联用对小细胞肺癌细胞NC I-H 69增殖的影响。方法通过脂质体将不同寡核苷酸导入NC I-H 69细胞中,分为反义寡核苷酸组、正义寡核苷酸组、无义寡核苷酸组和空白对照组4组,W estern B lot法检测细胞bcl-2蛋白的表达。不同浓度的VP 16作用于4组转染后的细胞,M TT法测定细胞存活分数。结果4组细胞中,与空白对照组相比较,反义寡核苷酸组的bcl-2蛋白表达明显受到抑制。bcl-2反义寡核苷酸转染细胞与VP 16作用后,反义寡核苷酸组细胞在各个浓度的存活分数均低于对照组,且有统计学意义(P<0.01)。结论bcl-2反义寡核苷酸与VP 16能协同抑制小细胞肺癌细胞的增殖。

Objective, To study the effect of bcl-2 antisense oligodexynucleotides combined with etoposide on human small cell lung cancer cell line NCI-H69. Methods, The different bcl-2 oligodexynucleotides was transfected into HCI-H69 cells using oligofectamine. Cultured cells were derided into 4 groups; antisense oligodexynucleotides （ASODN）, sense oligodexynucleotides （SODN）, nonsense oligodexynucleotides （NSODN） and control. The expression of bcl-2 was examined by Western Blot. Four group cells were incubated with different concentration etopcide respectively. The cell growth inhibition was assessed by MTT test. Results ：The bcl-2 expression in ASODN group was significantly inhibited compared with the control group. The survival fraction of ASODN group was significantly lower than that of the control group, and the difference had statistical significance. Conclusions,The bcl-2 ASODN combined with etoposide can enhance the inhibitory effect on small cell lung cancer cell.