长时间失血性休克大鼠肾脏糖皮质激素受体与磷酯酶A2改变及意义

Changes and significances of glucocorticoid receptor and phospholipase A 2 of renal tissue in long peroid hemorrhagic shock

用放射配体分析法，研究长时间失血性休克大鼠肾脏组织糖皮质激素受体（ＧＲ）变化并检测磷酯酶Ａ２（ＰＬＡ２）变化。结果显示：休克２小时后，ＧＲ的最大结合容量（Ｒ０）开始下降，并随休克时间延长而继续下降，于８小时后，ＧＲ的Ｒ０仅为对照组的２１％。对糖皮质激素（ＧＣ）的亲和力也下降。同时肾脏ＰＬＡ２上升，与ＧＲ的Ｒ０下降呈负相关（ｒ＝０．７１．Ｐ＜０．０５）。说明ＧＲ功能下降所致受体水平ＧＣ功能不全对长时间失血性休克的发生、发展及与休克后肾功能损害可能有关。

The glucocorticoid receptor(GR) of renal cytosol was determined in the long period hemorrhagic shock rats.Meanwhile,phospholipase A 2 (PLA 2) of renal tissue were tested.The results showed that 2 hours after shock,R 0 of GR decreased with the prolongation of shock.8 hours after shock,R 0 of GR was 21% of the control.The affinity to GC was decreased.Renal PLA 2 as an response index of GR was increased and the incrcase of PLA 2 was negatively correlated with the decrease of the Ro of GR.It is indicated that the dysfunction of GR might be related to the pathogenesis of hemorrhagic shock.