超声引导下细针穿刺在基因诊断胰腺癌中的应用

Gene Diagnosis of Pancreatic Adenocarcinoma by FNAB under the Guide of Ultrasound

采用超声引导下细针穿刺方法将ＰＣＲ—ＲＦＬＰ技术对３２例胰腺及其邻近器官细针穿刺标本进行Ｃ—ｋｉ—ｒａｓ等１２密码子突变应用于临床胰腺癌诊断。在２０例胰腺癌中１９例有Ｃ—ｋｉ—ｒａｓ第１２密码子突变，阳性率达９５％，而５例慢性胰腺炎，４例壶腹癌、３例胰岛细胞瘤等均无Ｃ—ｋｉ—ｒａｓ第１２密码子突变。结果说明超声引导下细针穿刺基因检测是诊断和鉴别诊断胰腺癌的安全有效方法。

Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)technique was used to analyse C-Ki-ras codon 12 mutation in 27 fine needle aspiration biopsy(FNAB) specimens of the pancreas and its adjacent organs under the guide of ultrasound for the diagnosis of pancreatic adenocarcinoma,C-Ki-ras codon 12 mutation was found in 19 out of 20 cases of pancreatic adenocarcinoma,the positive rate is 95%(19/20),whereas no mutation was found in those taken from 12 patients with chronic pancreatitis,carcinoma of ampulla'of vater and pancreatic cyst. The result shows that the detection of gene mutation with the technique of FNAB under the guide of ultrasound is a practical,sensitive,rapid and reliable method for the diagnosis of pancreaticcarcinoma.