补肾、活血、化痰三法的不同组合对拟血管性痴呆小鼠学习记忆及NO、NOS影响的比较研究

Comparative Studyof Effects on Learning Memory, NO and NOS of Vascular DementiaRats of Different Combinations of Kidney-invigorating, Blood-promotingand Phlegm-dissipating

目的：观察活血、补肾、化痰三法不同组合对拟血管性痴呆小鼠学习记忆及NO、NOS的影响，并进行比较性研究，筛选最佳治法组合。方法：复制脑部缺血再灌注拟血管性痴呆小鼠模型。术后15天检测各组小鼠行为学变化及脑组织内一氧化氮（NO）、一氧化氮合酶（NOS）的含量。结果：各给药组小鼠学习反应时间缩短，记忆潜伏时问延长，错误次数减少（P〈0．01），脑组织内NO、NOS含量明显降低（P〈0．05—0．01），其中以补肾活血化痰法疗效最为显著。结论：三法不同组合均能降低小鼠脑组织中NO、NOS含量，提高学习记忆能力，其中补肾活血化痰法是治疗VD的最佳疗法。

Objective： to observe the effects on learning memory, NO and NOS content of vascular dementia rats of different combinations of kidney-invigorating, blood - promoting and - phlegm-dissipating, and to compare the combinations to get the best one. Methods： vascular dementia model rats by cerebral isehemia and reperfusion were made. On the 15th day after operation, the ethological changes, NO and NOS in brain tissues were determined. Results： of rats in administration groups, learning reaction time shortened, memory latency lengthened, and mistakes reduced （ P 〈 0.01）; NO and NOS in brain tissues decreased significantly （ P 〈0.05 - 0.01）, with the method of kidney - invigorating, blood - promoting and phlegm - dissipating as the best. Conclusion： the different combinations can reduce the NO and NOS content in brain tissues and improve the ability of study and memory, and the method of kidney - invigorating, blood - promoting and phlegm - dissipating is the best therapy to VD.