睡眠剥夺对大鼠海马和皮质IL-1β蛋白表达的影响

The effects of total sleep deprivation on IL-1β protein expression in rats hippocampus and cortex

目的:研究完全睡眠剥夺对大鼠海马和皮质IL-1β蛋白表达的影响。方法:将大鼠放入1转/分钟转笼中,制作经历不同持续时间的完全睡眠剥夺(TSD)模型。30只大鼠随机分为5组:对照组(正常对照CC组,环境对照TC组)和睡眠剥夺组[TSD6h组,TSD1d组,TSD3d组(n=6只)]。采用免疫组化方法检测大鼠海马和皮质IL-1β蛋白表达。结果:IL-1β阳性细胞大多在细胞浆,胞核内也有表达;主要分布在海马CA3、CA1及齿状回细胞核和细胞浆,在大脑皮质分布于各层。结论:完全睡眠剥夺大鼠海马和皮质IL-1β蛋白表达增高,且随时间的延长而逐趋明显。睡眠短期剥夺,IL-1β蛋白表达增高可能对脑细胞具有应激性保护作用,而长期剥夺后IL-1β持续增高可能对神经元有损伤作用。

Objectives： To investigate the effects of total sleep deprivation on IL-1β protein expression in rat＇s hippocampus and cortex. Methods： Put the rats in the rotating drums whose rotating rate were 1/min to make the TSD model in different time course. Divided the 30 rats into total five groups randomly： antitheses group （CC group for normal and TC group for environment） and sleep deprivation group （TSD6h, TSDld, TSD3d （n= 6））. we adopt the approach of immunohistochemistry to test IL-1β protein expression in rat＇s hippocarnpus and cortex. Results： The positive ceils of IL-1β mostly distributed in cell plasm and the expression of IL-1β was also found in cell nucleus. It is mostly distributed in hippocampus CA3, CA1 and dentation gyrus nucleolus and cell plasm; and distributed in every stratum In cerebral cortex. Conclusions： Total sleep deprivation on IL-1β protein expression in rat＇s hippocampus and cortex was higher, and the longer the time course, the more significant the result was. The higher IL-1β protein expression maybe help to protect the brain cell after shorttime sleep deprivation, but they maybe harm the nerve cell after overtime in deprivation.