摘要
目的:研究完全睡眠剥夺对大鼠海马和皮质IL-1β蛋白表达的影响。方法:将大鼠放入1转/分钟转笼中,制作经历不同持续时间的完全睡眠剥夺(TSD)模型。30只大鼠随机分为5组:对照组(正常对照CC组,环境对照TC组)和睡眠剥夺组[TSD6h组,TSD1d组,TSD3d组(n=6只)]。采用免疫组化方法检测大鼠海马和皮质IL-1β蛋白表达。结果:IL-1β阳性细胞大多在细胞浆,胞核内也有表达;主要分布在海马CA3、CA1及齿状回细胞核和细胞浆,在大脑皮质分布于各层。结论:完全睡眠剥夺大鼠海马和皮质IL-1β蛋白表达增高,且随时间的延长而逐趋明显。睡眠短期剥夺,IL-1β蛋白表达增高可能对脑细胞具有应激性保护作用,而长期剥夺后IL-1β持续增高可能对神经元有损伤作用。
Objectives: To investigate the effects of total sleep deprivation on IL-1β protein expression in rat's hippocampus and cortex. Methods: Put the rats in the rotating drums whose rotating rate were 1/min to make the TSD model in different time course. Divided the 30 rats into total five groups randomly: antitheses group (CC group for normal and TC group for environment) and sleep deprivation group (TSD6h, TSDld, TSD3d (n= 6)). we adopt the approach of immunohistochemistry to test IL-1β protein expression in rat's hippocarnpus and cortex. Results: The positive ceils of IL-1β mostly distributed in cell plasm and the expression of IL-1β was also found in cell nucleus. It is mostly distributed in hippocampus CA3, CA1 and dentation gyrus nucleolus and cell plasm; and distributed in every stratum In cerebral cortex. Conclusions: Total sleep deprivation on IL-1β protein expression in rat's hippocampus and cortex was higher, and the longer the time course, the more significant the result was. The higher IL-1β protein expression maybe help to protect the brain cell after shorttime sleep deprivation, but they maybe harm the nerve cell after overtime in deprivation.
出处
《脑与神经疾病杂志》
2006年第2期102-104,共3页
Journal of Brain and Nervous Diseases
关键词
IL-1Β
免疫组化
睡眠剥夺
大鼠
海马
皮质
IL-1β
immunohistochemistry
sleep deprivation rat hippocampus cortex