柞蚕杂交F_1代的RAPD分析

Analysis to Genetic Change in F_1 Hybrids of Antheraea pernyi with RAPD Technique

利用分子标记技术对沈黄1号、选大1号及F1代进行了遗传分析。结果表明:卵期,正反交F1代的酯酶活性较两亲本的活性高,正反交F1代的酯酶活性OD值分别为0.6305,0.5515;酯酶同工酶电泳显示,正反交F1代的酶带宽、颜色深,表明F1代酯酶活性较强。采用RAPD技术,从30个引物中筛选出11个引物对4个柞蚕群体扩增出差异性条带,从F1代扩增出的非亲性位点及其基因组DNA较亲本具有更丰富的多态性两个方面,探讨了柞蚕杂种优势产生的遗传基础。

Molecular markers technique was used to analyze the genetic relationships among Shenhuang 1,xuanda 1 and their reciprocal hybrids in Antheren pernyi. Results showed that the optical density （OD）of esterase activity of reciprocal hybrids was 0.6305,0.5515 respectively, and higher than their parents＇. Eletrophoretic pattern of PAGEs had been showed that the bands were wider and darker in color than those from its parents. In a word, the hybrids＇ esterase activity was stronger. Using Random Amplified Polymorphic DNA（RAPD） technique with 30 kinds of random primers, 11 kinds of random primers were selected to the different bands in four groups of oak silkworm. From two sides, the bands not from their parents and the more abundant genes in their reciprocal hybrids, we discussed the molecular basis of heterosis effect in hybrids.