4300紫外可见分光光度计检测血中碳氧血红蛋白含量快速诊断一氧化碳中毒

Rapid Diagnosis of Carbon Monoxide Poisoning by 4300 Spectrophotometer

目的建立4300紫外可见分光光度计检测血中碳氧血红蛋白含量方法快速诊断一氧化碳(CO)中毒。方法检血用0.1%Na2CO3稀释,加入连二亚硫酸钠,4300紫外可见分光光度计测定500nm^600nm吸收光谱和538nm和555nm处吸光度值,确定碳氧血红蛋白,计算碳氧血红蛋白含量。结果氧和血红蛋白的最大吸收波长为555nm,碳氧血红蛋白的最大吸收波长为538nm和555nm。4300紫外可见分光光度计检测血中碳氧血红蛋白含量(10%~90%)回收率平均为95%~110%,标准差为2.1%~8.0%,最低检出浓度为2%。80例可疑CO中毒者中63例血中检出碳氧血红蛋白,平均含量为35%。168例可疑CO中毒死亡者中124例血中检出碳氧血红蛋白。结论4300紫外可见分光光度计检测血中碳氧血红蛋白,具有快速、准确、操作简便、结果可靠的特点,可应用于CO中毒的诊断和CO中毒死亡的法医学鉴定。

Objective To develop an identification and determination method of carbonylhaemoglobin in blood by 4300 spectrophotometer for rapid diagnosis of carbon monoxide poisoning. Methods Blood samples was diluted with 0.1% Na2CO3, to which sodium hydrosulfite was added. Its absorption band and absorption at 538 nm and 555 nm were determinate by a 4300 spectrophotometer. Results The absorption maximum wavelengths of andoxyhaemoglobin and carbonylhemoglobin were 555 nm and 538 nm, 555 nm. The mean recovery and standard deviation of carbonylhemoglobin in blood detected by the 4300 spectrophotometer were from 95% to 110% and from 2.1% - 8.0%. The limit of carbonylhemoglobin detection was 2%. Carbonylhemoglobin was identified in 63 out of 80 samples of doubtful carbon monoxide poisoning, and the mean blood carbonylhemoglobin content was 35%. Carbonylhemoglobin was identified in 124 out of 168. Conclusion This analysis was simpler, rapider, more sensitive and more practiced. It was best for diagnosis of carbon monoxide poisoning and forensic identification of death from carbon monoxide poisoning.