脑心通胶囊对拟血管性痴呆大鼠学习记忆及抗氧化作用的影响

Therapeutic Effects of Naoxintong Capsule on Rat Model with Vascular Dementia

目的研究脑心通胶囊对拟血管性痴呆(VD)大鼠的学习记忆行为及超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量的影响。方法选用纯系VD大鼠采用大脑中动脉梗死(MCAO)法制成VD大鼠模型后随机分为脑心通组(中药组)、西药组、模型组,另设假手术组和正常组,共治疗28d,治疗后以Morris水迷宫实验检测其学习记忆行为能力,并检测脑内SOD活性、MDA含量。结果与模型组比较,中药组和西药组均能提高Morris水迷宫成绩,提高SOD活力、降低MDA含量,反映中药组具有抗自由基损伤作用;与正常组相比,模型组脑组织的SOD活性显著下降,MDA含量显著增加,反映了自由基损伤明显,抗自由基损伤能力下降。结论脑心通胶囊可提高自由基清除酶的活力,减轻自由基损伤。

Objective To study the effects of Naoxintong capsule on behavior and hippocampal histomorphology in rats with vascular dementia. Methods The vascular dementia model was established by occlusion of the middle cerebral artery in rats. The faculty of learning and memory was evaluated by step - down avoidance test. The brain tissues were removedo Frozen sections were made and stained with hematoxylin-eosin（HE）. The hippoeampal histomorphology was observed and analyzed by light microcopy. Results The results of step down avoidance test showed that compared with sham - operated group, model group presented significantly the learning and memory impairments. Compared with model group, learning and memory improved in Naoxintong capsule group and Hydergine group. There was no significant difference between two therapy groups. Under the light microscope, hippocampal CA1 fields of rats in the sham - operated group exhibited closely and neatly spaced pyramidal cells, and insignificant neuron loss. Sparse and disturbed pyramidal cells, noticeable neuron loss and neuralgia cell proliferation could be seen in the hippoeampal CA1 fields of rats in the model group. Neuron loss reduced significantly in the hippocampal CA1 pyramidal cell fields in two therapy group and cell morphology presented relative normal. Conclusion Naoxintong capsule was effective for rat model with vascular dementia.