胃肠B细胞淋巴瘤中幽门螺杆菌的基因表型

Helicobacter pylori genotypes of gastrointestinal B cell lymphoma

目的:探讨原发胃肠B细胞淋巴瘤中幽门螺杆菌(Hp)毒力决定簇vac A(s1、s2、m2)、cag A及ice A 1的表达以及各致病基因间的相互关系。方法:采用免疫组化及PCR方法分析49例甲醛溶液固定石蜡包埋胃肠B细胞淋巴瘤标本。结果:49例胃肠B细胞淋巴瘤中黏膜相关淋巴组织淋巴瘤(MALT-L)14例(胃8例,肠6例),弥漫性大B细胞淋巴瘤(DLBCL)35例(胃21例,肠14例)。在MALT-L中Hp毒力决定簇vac A s1,vac A s2,vac A m2,ice A 1及cag A的表达分别为50．0％,42．9％,50．0％,14．3％和85．7％;在DLBCL中,分别为57．1％,40．0％, 25．7％,5．7％和91．4％。结论:含cag A基因的高毒力Hp菌株在胃肠B细胞淋巴瘤的发病中起一定的作用,而 Hp的vac A m2基因似乎与MALT-L关系更为密切。临床分期与各毒力基因无明确相关性。

Objective： To assess the expression of specific virulence-associated Helicobacter pylori （Hp） genotypes. （cag A, vac A, and ice A status） in primary gastrointestinal B cell lymphoma. Methotis： Forty-nine paraffin wax embedded tissues were analyzed by immunohistochemical staining and polymerase chain reaction. Results： There were 14 cases of mucosa associated lymphoid tissue lymphoma （MALT-L） （8 in stomach, and 6 in intestine） and 35 cases of diffuse large B cell lymphoma （DLBCL） （21 in stomach, and 14 in intestine） in all. The positive rates ofvac A s1,vac A s2,vac A m2,ice A 1 and cag A were 50.0% ,42.9% ,50.0%, 14.3% and 85.7% in MALT-L; 57.1% ,40.0% ,25.7%, 5.7% and 91.4% in DLBCL, respectively. Conclusion： High toxicity cag A ＋Hp strains, seem to play a role in the pathogenesis of gastrointestinal B cell lymphoma, whereas vac A m2 may be more associated with MALT-L. Clinical stage was not associated with virulence-associated Hp genotypes.