摘要
目的:研究毒物鱼藤酮和1-甲基-4-苯基吡啶离子(MPP+)对野生型及转染囊泡单胺转运蛋白(VMAT2)基因的中国仓鼠卵巢细胞(CHO)的毒性作用。方法:将不同浓度的MPP+、鱼藤酮与野生型CHO细胞(WT-CHO)和转染VMAT2基因的CHO细胞(VMAT2-CHO)共同培养,采用细胞MTT比色法和形态学观察,探讨毒物对细胞的毒性作用,通过激光共聚焦显微镜观察毒物对2种细胞的细胞内钙浓度([Ca2+]i)的影响。结果:VMAT2-CHO在一定时间(72 h)内对MPP+(0.2-2.0 mmol/L)和鱼藤酮(0.05-1.0μmol/L)的毒性有抵抗作用(P<0.05)。结论:VMAT2可以抵抗一定浓度的MPP+和鱼藤酮引起的神经细胞毒性作用。
Objective： The toxicity to transgenic CHO of vesicular monoamine transporter 2 （VMAT2） and to wild type CHO generated by MPP^＋ and Rotenone was assessed, Methods： MTT assay was used to detect cell survival rate of both WTCHO and VMAT2-CHO following exposure to MPP^＋ and Rotenone, Meanwhile the change of cell morphology was observed, Fluorescence intensity of intracellular Ca^2＋ concentration in the two groups of cells was measured under the Confocal laser microscopy, Results：Transgenic VMAT2-CHO cells were resistent to the toxicity of MPP^＋ （ the concentration range： 0.2 2.0 mmol/L） and Rotenone（ the concentration range： 0.05-1. 0μmol/L）. Conclusion： Transgenic CHO cells with VMAT2 can resist the toxicity caused by Rotenone and MPP^＋ .
出处
《神经损伤与功能重建》
2006年第1期31-34,共4页
Neural Injury and Functional Reconstruction
基金
河北省自然科学基金资助项目(303497)
