共固定化生产高含量低聚果糖的研究

Production of High-purity Fructo-oligosaccharides with Co-immobilized As per gillus niger and Enzyme

生产低聚果糖过程中，副产物葡萄糖抑制酶反应，降低了产物中低聚果糖的含量。本文采用两种固定化方法，通过去除或转化葡萄糖，从而制备出高含量的低聚果糖。首先以２％戊二醛和０．１％丹宁于２０℃时处理固定化黑曲霉菌体与葡萄糖氧化酶，将菌体与酶共包埋得到固定化颗粒，所得到的共包埋产物于５０℃ｐＨ５．０条件下与５０％蔗糖溶液摇瓶反应２４ｈ，得到７１％的低聚果糖；又采用固定化黑曲霉增殖细胞与固定化葡萄糖异构酶协同作用方法，将５０％蔗糖溶液通入柱式反应器，连续生产得到高含量低聚果糖，产物中低聚果糖和果糖含量分别为６３％和１６％。

he fructosyltransfer reaction of sucrose could be inhibited by glucose which was a by-product in the production of fructo-oligosaccharides(FOS).Removal or isomerizing of such glucose would make it possible to produce high-purity FOS. High-purity FOS was prepared by two processes of converting glucose with glucose oxidase (GOD) and immobilized isomerase (IGI). In former process,GOD was crosslinked with 2% glutaradehyde and 0. 1% tannin and co-entrapped with Aspergillus niger in calcium alginate gel. After the co-immobilized beads were incubated at PH 5. 0, 50℃ for 24 hours, 71% FOS was prepared.In latter one immobilized As pergillus niger and IGI were co-packed in a columm and 63% FOS and 16% fructose were produced.