摘要
生产低聚果糖过程中,副产物葡萄糖抑制酶反应,降低了产物中低聚果糖的含量。本文采用两种固定化方法,通过去除或转化葡萄糖,从而制备出高含量的低聚果糖。首先以2%戊二醛和0.1%丹宁于20℃时处理固定化黑曲霉菌体与葡萄糖氧化酶,将菌体与酶共包埋得到固定化颗粒,所得到的共包埋产物于50℃pH5.0条件下与50%蔗糖溶液摇瓶反应24h,得到71%的低聚果糖;又采用固定化黑曲霉增殖细胞与固定化葡萄糖异构酶协同作用方法,将50%蔗糖溶液通入柱式反应器,连续生产得到高含量低聚果糖,产物中低聚果糖和果糖含量分别为63%和16%。
he fructosyltransfer reaction of sucrose could be inhibited by glucose which was a by-product in the production of fructo-oligosaccharides(FOS).Removal or isomerizing of such glucose would make it possible to produce high-purity FOS. High-purity FOS was prepared by two processes of converting glucose with glucose oxidase (GOD) and immobilized isomerase (IGI). In former process,GOD was crosslinked with 2% glutaradehyde and 0. 1% tannin and co-entrapped with Aspergillus niger in calcium alginate gel. After the co-immobilized beads were incubated at PH 5. 0, 50℃ for 24 hours, 71% FOS was prepared.In latter one immobilized As pergillus niger and IGI were co-packed in a columm and 63% FOS and 16% fructose were produced.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
1996年第1期1-7,共7页
Food and Fermentation Industries
关键词
共固定化
低聚果糖
黑曲霉
Fructo-oligosaccharides, Co-immobilization, As pergillus niger