乳腺癌组织芯片中BAG-1和p73及p16的表达及其意义

Expressions and significance of BAG-1,p73 and p16 in breast cancer tissue microarray

目的:检测BAG-1、p73和p16在乳腺癌、癌旁组织和正常组织中的表达,并讨论其意义。方法:利用组织芯片采用免疫组化DAKO Envision法检测乳腺癌、癌旁组织、正常组织中BAG-1、p73和p16的表达。结果:组织芯片中BAG-1、p73和p16在胞核、胞质均有表达,在正常组织中以胞质为主,但随着病变表达逐渐增加。在乳腺癌组织中的表达比乳腺癌癌旁组织、正常组织中高,差异有统计学意义,P<0·01;而p16的表达正好相反,差异亦有统计学意义,P<0·01。BAG-1、p73和p16的阳性率在Ⅰ级和Ⅱ、Ⅲ级乳腺癌之间差异无统计学意义,P>0·05。结论:BAG-1、p73和p16表达的变化提示它们可能参与了乳腺癌的发生。

OBJECTIVE：To detect BAG-1, p73, p16 expressions in breast cancer by tissue microarray and evaluate their clinical significance. METHODS： Tissue mieroarray of breast cancer, atypoical hyperplasia and normal tissues were analyzed by DAKO Envision system immunohistochemical staining for BAG-1, p73 and p16 expressions. RESULTS： BAG-1, p73 and p16 were expressed in nucleus and cytoplasm in cancer tissue, they were mainly existed in cytoplasm in normal epithelium. With the change procedure from the normal cell to dysplasia to cancer tissue, the degrees of BAG-1 and p73 increased gradually, while the degree of p16 decreased. There were significant differences of positive rates of BAG-1, p73 and p16 between the cancer tissue and the normal,P〈0. 01. but there were no significant differences of positive rates of BAG-1, p73 and p16 between the gradeⅠand gradeⅡ, Ⅲcancer tissues, P〉0. 05. CONCLUSION： There are overexpressions of BAG-1, p73 and p16 in breast cancer. It suggests that BAG-1, p73 and p16 may be related to breast cancer.