Survivin基因在胃癌中表达的研究

Survivin gene expression in gastric carcinoma

目的研究凋亡抑制基因survivin在胃癌组织中的表达。方法运用S-P免疫组化技术,检测survivin基因在93例胃癌组织、20例正常胃黏膜组织以及胃癌细胞株MGC-803中的表达。结果Survivin基因在20例正常胃黏膜组织中不表达,而93例胃癌组织中有66例表达阳性,占71%,两者差异有统计学意义(P<0.01),胃癌细胞株MGC-803中survivin呈高表达。Survivin基因表达阳性率与胃癌组织的分化程度、淋巴结转移、TNM分期相关,而与浸润程度不相关。结论Survivin基因的特异性高表达在胃癌的发生发展中起重要作用,其过度表达可能提示胃癌预后不良。

Objective To detect the expression of a novel inhibitor gene of apoptosis, survlvin in gastric carcinoma tissues and in gastric carcinoma cell line MGC- 803. Methods In 93 gastric carcinoma tissues and 20 no - neoplastic gastric tissues, the expression of survivin gene was examined by using streptavidin- biotin peroxidase （SP） method. Results In contrast to no expression of survivin in no-neoplastic gastric mucosa tissues, survlvln was expressed in 66 of 93（71% ） cases of gastric cancer samples, and overexpresslon of survivin in gastric carcinoma cell line MGC - 803 was also presented. Survivin gene expression was related to the differentiation, lymph node metastase and the TNM stage of gastric carcinoma. Conclusions The specifically up- regulation of survivin gene in gastric cancer indicates that survivin may play an important role in the process of carcinogenesis.