EGF对体外培养食管癌细胞增殖及迁徙能力的影响

Growth inhibitory and invasive effect of EGF on esophageal cancer cells in vitro

目的：观察不同浓度表皮生长因子（epidermal growth factor，EGF）对食管癌细胞株EC109和EC-1增殖及漫润能力等生物学特性的影响。方法：观察细胞形态，MTT检测细胞增殖活性，单克隆扩散实验和划痕损伤实验检测细胞的浸润迁徒能力，免疫荧光观察细胞骨架的组分微丝的改变。结果：高浓度组2株食管癌细胞形态发生上皮细胞-间叶样变（epithelial-mesenchymal transition，EMT）；0．01～0．1μg／LEGF对EC109和EC-1食管癌细胞株均有轻微的抑制增殖效应，1～100μg／LEGF能明显卿制EC109和EC-1细胞的增殖，其中EGF的最大抑制效应浓度为10μg／L；单克隆扩散实验和划痕损伤实验表明，高浓度组均有细胞迁出；免疫荧光提示，高浓度EGF能诱导β-actin重新分布，细胞的长极出现较长的应力纤维束。结论：高浓度EGF对食管癌细胞株EC109和EC-1的增殖有一定的抑制作用，并且能促进食管癌细胞发生迁徒，诱导微丝发生明显重组。

OBJECTIVE：To study the effect of EGF on esophageal cancer cell lines EC109 and EC-1 in culture. METHODS： The morphological changes were observed by using phase microscope; relative cell viability was evaluated by MTT assay, and the mobility and invasion evaluated by the scattering assay and wound-healing assay; the cytoskeleton was observed by the immunofluorescence. RESULTS： EGF induced cells epithelial-mesenchyme transition （EMT）. At the concentration of 0.01--0. 1 μ/L,EGF had slight growth inhibitory effect on EC109 and EC-1, at the concentration of 1 -- 100μg/L, EGF remarkably inhibited that the growth of them, and the maximal inhibitory concentration was 10μg/L. Scattering assay and wound-healing assay indicated the ability of migration was improved at high concentrations. EGF also induced redistribution of β-actin, and there were a few bundles of stress filaments running the whole length of the cell body. CONCLUSION： At the high concentrations, EGF can inhibit the growth of EC109 and EC-1, and at the same time, they can improve migration and induce reorganization of the microfilament systern.