HPLC法测定人血浆中西洛他唑的浓度

Determination of cilostazol in blood plasma by HPLC

目的建立测定人血浆中西洛他唑浓度的HPLC法。方法以迪马公司钻石C18反相柱（250mm×4．6mm，5μm）为色谱柱，流动相为甲醇-0．03mol·L^-1醋酸铵（62：38，V/V），流速0．8mL·min^-1，检测波长257nm，以乙酸乙酯为提取剂。结果西洛他唑高（1000μg·L^-1）、中（500μg·L^-1）、低（25μg-L^-1）3种质量浓度的平均回收率分别为99．51％、97．07％、98．28％，日内、日间RSD均〈7％；分析方法的最低检测浓度为10μg-L^-1。线性范围为10—1500μg·L^-1。结论该方法灵敏、准确、简单、快速，可用于西洛他唑临床血药浓度监测和药动学研究。

AIM To develop a HPLC method for quantitative determination of cilostazol in blood plasma. METHODS Blood plasma samples were extracted with hexane to remove lipid and interfering substances, then extracted with ethyl acetate. The residue was analyzed with a reverse phase HPLC system （C18 column , 250 mm× 4.6 mm,5 μm）, with mobile phase of MeOH-0.03 mol.L^-1 NH4 AC （62：38, V/V）, UV detection wave length of 257 nm , flow rate of 0.8 mL.min^-1. RESULTS The average recoveries for cilostazol were 99.51%, 97.07% and 98.28% ,respectively. The within-day and between-day relative standard deviations were lower than 7 %. The calibration curves had good linearity （r = 0.999 9） within a concentration range of 10 - 1 500 μg.L^-1. The limit of quantitation for cilostazol was 10 μg.L^-1. CONCLUSION The method provides a sensitive , accurate , precise and reliable analytical procedure for clinical monitoring of cilostazol blood plasma and its phamacokinetic studies.