北豆根提取物抗突变和致突变的实验研究

Experimental study on the antimutagenic and mutagenic activity of Rhizoma menispermi extracts

目的:分析中药北豆根不同提取物的抗突变和致突变作用,为抗肿瘤新药开发、研制提供依据。方法:用三步法初步分离、纯化北豆根提取物,得到北豆根水提液(aqueousextractofrhizomamenispermi,RMAE)、醇提液的初步纯化产物蝙蝠葛酚性生物碱(phenolicalka-loidsofmenispermumdauricum,PAMD)和北豆根多糖成分(rhizomamenispermipolysaccha-ride,RMP)。采用细菌培养技术进行抗突变致突变直接及间接同步快速实验,以检测不同北豆根提取物的抗突变和致突变活性。结果:北豆根不同提取物RMAE、PAMD和RMP的直接和间接致突变实验结果均为阴性。而RMP直接和间接抗突变结果为阳性,并且具有浓度依赖性。RMAE、PAMD直接和间接抗突变实验结果均为阴性。结论:北豆根各提取物均无致突变毒性,而RMP具有直接和间接的抗突变活性。

OBJECTIVE： To investigate the antimutagenic and mutagenic activity of the extracts from Rhizoma menispermi, a Chinese medicinal herb in order to provide the scientific methods for exploring new antitumor drugs. METHODS： An aqueous extract of Rhizoma menispermi （RMAE）, an alcohol purified extract named phenolic alkaloids of menispermum dauricurn （PAMD） and Rhizoma menisperrni polysaccharide （RMP） were isolated and purified by using the three-step method. The antimutagenic and mutagenic activities of the three Rhizoma menispermi extracts were studied under the direct and indirect rapid synchronous test by using the bacterium culture technique. RESULTS： The direct and indirect mutagenic tests showed that the results of all Rhizoma menipermi extracts （RMW,PAMD and RMP） were negative; both direct and indirect antimutagenic tests indicated that the results of RMP were positive. RMW and PAMD were negative and RMP expressed strong positive reaction in a concentration-dependent manner. CONCLUSION： All agents of Rhizoma menipermi extracts have no mutagenie activity; RMP has direct and indirect antimutagenic activity.