ERCP术后胰腺炎的动物模型制备

An alternative method of establishing a post-ERCP pancreatitis animal model

目的探讨合理制备ERCP术后胰腺炎的动物模型方法。方法18只日本大耳兔随机分为3组,每组6只。A组:胰管内注射30%泛影葡胺0.5ml/kg体重+胰管阻塞;B组:胰管内注射生理盐水0.5ml/kg体重+胰管阻塞;C组:正常对照,开腹后翻动肠管即关腹。测定术后8h及24h的血淀粉酶、脂肪酶,同时对术后24h的胰头组织进行病理组织学分析。结果与C组和B组相比,A组术后8h及24h的血淀粉酶、脂肪酶显著升高(P<0.05);术后24h胰头部分的病理改变主要表现为明显的水肿和炎细胞浸润,仅有轻度的出血坏死,病变范围<50%,多数在30%以内(P<0.05);表现为急性水肿性胰腺炎。结论采用30%泛影葡胺0.5ml/kg体重兔胰管内逆行注射+胰管阻塞的方法能复制急性胰腺炎的动物模型,该模型稳定可靠,其发病机制类似于临床上的ERCP术后胰腺炎。

Objective The aim of this study was to explore an alternative method of establishing a post-ERCP pancreatitis animal model. Methods 18 rabbits were equally randomized to 3 groups. The animal models were induced by retrograde infusion of either 30% Urografin or saline solution 0.5 ml/kg into the pancreatic duct of rabbits plus ligation. The control group was induced by sham operation only. Serum amylase and lipase were determined at 8 and 24 hours, and histopathologic changes in the head of pancrease were evaluated at 24 hours. Results Compared with saline solution and control groups, serum amylase and lipase decreased significantly in 30% Urografin group at 8 and 24 hours （P 〈 0.05）, where obvious edema of the head of pancreas, infiltration of inflammatory cells, mild necrosis and hemorrhage were observed. The involvement of pathological change was mostly 〈 30%, and 〈 50% in some cases, presenting as acute pancreatitis. Conclusions Retrograde infusion of 30% Urografin into the pancreatic duct of rabbits plus obstruction can successfully imitate a stable and reliable animal model of acute pancreatitis similar to clinical post-ERCP pancreatitis.