新型犬脊柱侧弯动物模型的建立

Creation of dog scoliosis model

目的 以较小损伤脊柱结构的方法建立进展性、结构性犬胸腰段脊柱侧弯动物模型。方法 健康杂种犬，采用右侧第8．13肋离断，左侧第8-13肋胸科钢丝固定，并与L2棘突相连加压的方法，建立脊柱侧弯模型。术后当日及1、3个月分别行脊柱X线检查，对已诱导成功犬随机选取2只行CT及病理学检查，记录并观察侧弯情况。结果 20只犬中17只诱导侧弯成功，成功率85％。Cobb角从术后当日平均34．48°，术后3个月进展为平均58．76°，平均进展24．28°，差异有显著性（P〈0．01）。CT示椎体旋转，组织学检查示顶椎椎体楔形变。结论 该造模方法成功建立大型动物脊柱侧弯模型，且最大程度避免损伤脊柱及其附属结构，成功率高，重复性好，为进一步实验创造了条件。

Objective To found a progressive and structural thoracolumbar scoliosis model with less trauma. Methods The hybrid dogs were undergone right 8 - 13 ribs osteotomies, and left 8 - 13 ribs stainless wire tethering and binding with L2 spinal process. Roentgenography was performed immediately, 1 day, 1 and 3 months after operation. For those with successful model creation, 2 were undergone CT and pathological examinations, and the data about scoliosis were recorded. Results 17 out of 20 got scoliosis, the successful rate was 85%. Cobb angle was 34. 48° immediately on average, and was processed to 58.76° in 3 months, which increased 24. 28°（P 〈0. 01 ） , which was significant different. Spinal rotation was found in CT. The apex vertebra was found to be wedge shaped in histological study. Conclusions With this method, big animal scoliosis model can be made, which can mostly avoid injury to spine and affiliated structure. It has high rate success and good repeatability, which provides condition for further study.