神经生长因子在大鼠骨折部位的表达

Expression of nerve growth factors in the fractured bone of rats

目的:用免疫组织化学方法来观察神经生长因子在大鼠骨折和非骨折部位的定位,分析神经生长因子在促进骨折修复中的可能作用。方法:实验于2005-06/11在大连大学附属中山医院骨科实验室完成。①取SD大鼠24只随机分为实验组18只,对照组6只。另取1只大鼠取其下颌下腺作为免疫组织化学的阳性对照。②实验组SD大鼠在第6肋距脊柱1cm处剪断肋骨建立肋骨骨折模型,对照组只暴露肋骨不切断。③实验组分别于术后1,3,6周各取6只大鼠在麻醉状态下取骨折部位1/6肋骨,对照组每次取2只,方法、部位同实验组,进行免疫组织化学染色检测神经生长因子。结果:25只全部进入结果分析。①对照组(非骨折部位)的肋骨,只有骨膜的间质细胞及骨骼肌纤维显示神经生长因子轻度着色。②实验组第1,3周,骨折部位的骨母细胞、骨髓基质细胞、成骨细胞染色阳性,大多数软骨细胞和骨痂也被染色。在骨折后6周,骨膜骨母细胞染色阳性。在非骨折肋骨,骨膜骨母细胞染色阳性。骨折部位,骨母细胞、骨髓基质细胞、成骨细胞、某些软骨细胞、骨膜基质细胞和骨骼肌细胞染色阳性。结论:①神经生长因子对正常骨组织的神经维持有重要作用。②神经生长因子对骨的新陈代谢和骨折的修复通过神经系统起促进作用。③神经生长因子对骨修复可能有直接的作用。

AIM： To observe the location of nerve growth factor in the fractured and non-fractured bone with immunohistochemcal method, and to analyze the possible effect of nerve growth factor in p.romoting the reparation of bone. METHODS： This experiment was carried out at the Laboratory of Orthopaedics, Zhongshan Hospital Affiliated to Dalian University from June 2005 to November 2005. ①Twenty-four SD rats were chosen and randomly divided into experimental group （n=18） and control group （n=6）. Another rat was chosen and its submandibular gland was used as immunohistological positive control. ②Rib was cut off 1 cm away from vertebral column on the 6^th rib of the SD rat in the experimental group, and only rib was exposed , but not cut in the control group. ③Six 1/6 rib was respectively chosen from the fractured bone under anaesthetic status at postoperative 1, 3 and 6 weeks in the experimental group. Two 1/6 rib was chosen in the control group, the method and part were the same as that in the experimental group. Immunohistochemical staining was used to detect nerve growth factor. RESULTS： Totally 25 rats entered the stage of result analysis. ①In the ribs of control group （non-fractured part） , only mesenchymal cells of periosteum and skeletal muscle fiber showed nerve growth factor stained lightly. ②At the first and third weeks in experimental group, osteoprogenitor cells, marrow stroma cell and osteoblasts of fractured part were stained positive. Most cartilage and bony callus were also stained. At the sixth week after fracture, osteoprogenitor cells of periosteum were stained positive. In the non-fractured rib, osteoprogenitor cells of periosteum were stained positive. In the fractured part, osteoprogenitor cells, marrow strema cell, osteoblasts, some cartilages, periosteum stroma cells and skeletal muscle cells were stained positive. CONCLUSION： Nerve growth factor plays an important role in the nerve maintaining of normal bone tissue, promotes the metabolism of bone and the reparation of bone fracture through nerve system, and directly irtfluences bone reparation.