人脐血干细胞定向分化为神经细胞的诱导方法

Neural differentiation of human umbilical cord stem cells

目的:分析人脐血干细胞在体外和体内向神经细胞分化的不同诱导条件。资料来源:应用计算机检索Medline数据库1990-01/2005-10相关脐带血干细胞向神经细胞分化文章,检索词为“umbilicalcordblood,mesenchymalstemcells,neuraldifferentiation”,分别组合进行检索,限定文章语言种类为English,同时计算机检索中文期刊数据库2000-01/2005-10相关脐带血间充质干细胞向神经分化文章,检索词为:“脐带血,间充质干细胞,神经分化”,分别组合进行检索,限定文章语言种类为中文。资料选择:初审资料,选取包括人脐血干细胞分化为神经细胞的文章。纳入标准:①脐带血间充质干细胞。②间充质干细胞向神经细胞分化。排除标准:①脐带血造血干细胞。②文献综述,重复研究,Meta分析类文章。资料提炼:共收集到68篇符合要求的文章,查找全文,选取其中近期相关文章19篇。将所有文章按照脐血干细胞诱导分化体外实验和体内实验进行分类综合。资料综合:脐带血干细胞来源丰富、采集方便、安全,且体外扩增迅速,在体外和体内一定条件下可以分化为神经细胞,然而不同的诱导条件对其分化的影响有不同,人们运用各种手段希望能找到诱导效率较高的诱导方法,包括几种化学诱导剂联合,生长因子之间配伍,化学诱导剂与生长因子联合,或应用接近生理状态的条件培养基等。在改进诱导条件的同时人们开始关注单一表型的细胞群作为实验对象是否能有效地提高诱导效率。研究者以期找到一种高效率而具有应用价值的诱导方法,为将脐带血干细胞应用于神经科学领域提供有力的证据。结论:人脐血干细胞在体外和体内一定条件下可以分化为神经细胞,应用于中枢神经系统疾病的细胞治疗具有广阔前景,然而寻找高效实用的诱导方法仍需要进一步研究。

OBJECTIVE： To study different conditions in neural differentiation of human umbilical cord blood in vitro and in vivo. DATA SOURCES： The English literatures of neural differentiation of human umbilical cord blood cells which were published between January 1990 and December 2005 were collected from the Medline by using the key terms ＂umbilical cord blood and mesencbymal stem cells,neural differentiation＂. Respectively the Chinese literatures which were published between January 1990 and December 2005 were collected, from the Chinese journals full-text database by using the key terms ＂umbilical cord blood and mesencbymal stem cells,neural differentiation＂. STUDY SELECTION： All articles were selected firstly, and those related to neural differentiation of human umbilical cord blood were selected. Inclusive criteria： ①The umbilical cord blood and mesenchymal stem cells. ②Neural differentiation of mensenchymal stem cells. Exclusive criteria： ①Haemopeietic stem cells from umbilical cord blood ②Review , repetitive study or Meta analytical papers. DATA EXTRACTION： A total of 68 articles meeting the criteria were collected and their full texts were looked up. 19 related articles published recently were collected. All the articles were classified into in vitro experiment and in vivo experiment according to induction and differentiation of human umbilical cord blood stem cells. DATA SYNTHESIS： Human umbilical cord blood stem cells are abundant in source, easy and safe in collection. And its in vitro proliferation is fast and it can differentiate into nerve cells under certain conditions in vitro and in vivo. However, different induction conditions affect its differentiation differently. People try to find the high-efficiency induction methods, including the combination application of several inducers, compatibility of several growth factors, combination of chemical inducer and growth factor or the application of condition culture medium which are close to psychological status and so on. When the induction conditions were improved, people began to focus on whether the single phenotypic cell population as experimental objects can improve the induction efficiency or not. We try to find a high-efficiency and valuable induction method, so as to provide reasonable basis for the application of human umbilical cord blood stem cells in the neuroscientific field. CONCLUSION： The stem cells derived from human umbilical cord blood can differentiate into nerve ceils under certain conditions in vitro and in vivo. It has wide prospect in cell therapy of central nervous system disease. While further study on high-efficiency and practical induction method should be given.